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以水稻成熟种胚为外植体,研究了不同类型水稻体细胞组织培养的辐射生物学效应,γ射线与NaN_3复合处理的体细胞培养反应,以及水稻花药愈伤组织的γ辐射效应。结果表明,各品种的出愈率、愈伤组织生长率的辐射剂量效应曲线可以用多靶单击模型拟合。不同类型品种的体细胞培养辐射效应存在着明显的差异。小剂量(100Gy,150Gy)时,有促进绿苗分化的作用。经γ射线加NaN_3复合处理,明显降低种子出愈率、初代培养愈伤组织生长率和分化率,其处理效应高于单独处理。但第2、3次培养时,γ射线处理效应大于γ射线加NaN_3复合处理效应。在以水稻成熟种胚作外植体时,培养前采用200Gy~(137)Csγ射线加2mmolNaN_3进行复合处理是可行的。水稻花药愈伤组织经30Gyγ射线处理可提高连续培养的绿苗分化率。
Using the mature embryo of rice as explants, the radiobiological effects of different types of rice somatic cell cultures, the somatic cell culture responses of γ-rays and NaN_3, and the γ-radiation effects of the anther callus of rice were studied. The results showed that the dose-response curves of the callus emergence rate and the callus growth rate of each variety can be fitted by a multi-target click model. Different types of somatic cell culture radiation effects there is a clear difference. Small doses (100Gy, 150Gy), there is to promote the role of green shoot differentiation. The γ-ray and NaN_3 composite treatment significantly reduced the seed out of the rate of primary culture callus growth rate and differentiation rate, the treatment effect is higher than the single treatment. However, in the second and third cultivation, the γ-ray treatment effect was greater than the γ-ray and NaN_3 compound treatment effect. When mature embryos of rice were used as explants, it was feasible to use 200 Gy ~ (137) Csγ ray and 2 mmolNaN_3 before culturing. The anther callus of rice was treated with 30 Gy γ-rays to improve the rate of green shoot differentiation in continuous culture.