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通过扫描电镜观察芝麻苗期叶片表面腺毛的显微结构,以二氯甲烷作为溶剂提取腺毛分泌物,并用GC/MS鉴定正常和干旱条件下不同芝麻品种叶片腺毛次生代谢物的差异。结果显示,苗期芝麻叶片表面存在非腺体腺毛、长柄腺毛、短柄腺毛及无柄黏液毛等多种腺毛类型,叶表面气孔属于毛茛型;芝麻叶腺毛分泌物包含多种组分,其中峰面积>2%的组分是主要代谢物,主要由酯类及直链饱和烷烃物质组成,且品种间具有显著差异;干旱条件下,分泌物的组分显著改变,抗旱性较强的冀9014有10个组分的相对含量高于其他品种,这些组分包含三十六烷与三十四烷类物质。上述结果表明,用GC/MS鉴定芝麻叶表面腺毛分泌物是可行的,分泌物组分可以反映品种间的差异,并能揭示干旱条件对芝麻代谢的影响。可以认为,芝麻腺毛与腺毛分泌物组分在芝麻种质资源鉴定及抗旱种质选育中具有重要的应用价值。
The microstructure of glandular hairs on the leaf surface of sesame seeds was observed by scanning electron microscopy. The glandular hairs secretions were extracted with methylene chloride as solvent, and the differences of secondary metabolites of glandular trichomes in different sesame cultivars under normal and drought conditions were identified by GC / MS. The results showed that there were many glandular glandular types such as glandular glandular trichomes, long glandular trichomes, short glandular glandular trichomes and sessile mucilage on the surface of sesame leaves at seedling stage. The stomata on leaf surface were of buttercup type. The sesame leaf glandular hair secretions contained A variety of components, including peak area> 2% of the components is the main metabolites, mainly composed of esters and straight-chain saturated alkanes composition, and significant differences between varieties; drought conditions, the composition of secretions significantly changed, The more drought-resistant Ji 9014 ten components of the relative content of higher than other varieties, these components include trihexadecane and tetradecane substances. The above results indicate that it is feasible to identify glandular hairy exudates on the surface of sesame leaves by GC / MS. The components of secretions reflect the differences among cultivars and reveal the effects of drought on the metabolism of sesame. It can be considered, sesame glandular hair and glandular hair secretions components in sesame germplasm identification and drought resistance germplasm breeding has an important application value.