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目的:建立HPLC同时测定糖敏灵丸中芍药内酯苷、芍药苷、柚皮苷、新橙皮苷及黄芩苷含量的方法。方法:采用Aglient ZorbaxSB-C18色谱柱(4.6 mm×250 mm,5μm),以0.05%磷酸水溶液-乙腈为流动相进行梯度洗脱,流速1 mL·min-1,检测波长230 nm,柱温30℃。结果:芍药内酯苷、芍药苷、柚皮苷、新橙皮苷及黄芩苷的线性范围分别为0.008～0.320,0.021～0.832,0.088～3.52,0.124～4.96,0.086～3.44μg(r≥0.999 9,n=7);平均回收率(n=6)分别为99.7%(RSD2.0%),100.1%(RSD 1.8%),100.1%(RSD 1.5%),100.0%(RSD 2.4%),103.6%(RSD 2.4%)。结论:所建立的HPLC方法简便,结果准确、可靠,重复性好。
OBJECTIVE: To establish a HPLC method for the simultaneous determination of albiflorin, paeoniflorin, naringin, neohesperidin and baicalin in Tangyelin pills. METHODS: Aglient Zorbax SB-C18 column (4.6 mm × 250 mm, 5 μm) was used as the mobile phase with a gradient of 0.05% phosphoric acid in acetonitrile as the mobile phase at a flow rate of 1 mL · min-1. The detection wavelength was 230 nm and the column temperature was 30 ℃. Results: The linear ranges of paeoniflorin, paeoniflorin, naringin, neohesperidin and baicalin were 0.008-0.320,0.021-0.832,0.088-3.52,0.124-4.96,0.086-3.44μg (r≥0.999 (RSD 2.0%), 100.1% (RSD 1.8%), 100.1% (RSD 1.5%), and 100.0% (RSD 2.4% 103.6% (RSD 2.4%). Conclusion: The established HPLC method is simple, accurate, reliable and reproducible.