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目的 探讨山东省汉坦病毒 (HV)主要流行株的基因型和流行特点。方法 采集山东地区有代表性的鼠肺标本 ,应用逆转录聚合酶链反应 (RT -PCR)扩增技术及核苷酸序列测定方法对免疫荧光抗原阳性的标本进行HVG2基因扩增并测序 ,与原有山东毒株一起进行同源性分析、系统发生树分析。结果 山东省新分离 18株HV均为汉城 (SEO)型 ,与以往山东SEO型HV有较高同源性 ,在 95 . 7%以上 ,属于同一亚型。山东省汉滩 (HTN)型HV同源性较低 ,在 77. 9%以上 ,属于不同亚型。结论 山东省HV是以SEO型为主的SEO和HTN混合型疫区。SEO型基因变异率低 ,有较高的稳定性。而HTN型HV变异率高 ,稳定性差。
Objective To investigate the genotype and epidemiology of Hantaviruses (HV) in Shandong Province. Methods Rat lung samples collected from Shandong Province were collected. The HVG2 gene was amplified and sequenced by RT-PCR and nucleotide sequencing. The original strain of Shandong homology analysis, phylogenetic tree analysis. Results The 18 new HV isolates in Shandong Province were both Seoul (SEO) type and higher homology with Shandong SEO type HV in the past, accounting for more than 95.7% and belonging to the same subtype. Hantaan (HTN) type HV in Shandong province has a low homology of more than 77.9%, belonging to different subtypes. Conclusion Shandong Province HV is a SEO-based SEO and HTN mixed-type epidemic area. SEO gene mutation rate is low, high stability. HTN HV mutation rate is high, poor stability.