Optimization of the Enzyme Matching Ratio by the Mixture Design in the Extraction of Lonicera edulis

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[Objective] The research aimed to find the optimum proportions of pectase,cellulase and papain in the extraction of Lonicera edulis pigment.[Method] The influences of different proportions of three enzymes on the absorbance value of Lonicera edulis pigment extraction solution were studied by the mixture design.The regression model between the enzyme matching ratio and the absorbance value was established,and the interaction effects of three enzymes were studied.Based on the model optimization,the optimum ratio was obtained.[Result] The optimum matching ratios of three enzymes were the pectase 46.6%,cellulase enzyme 17.6% and papain 36.0%.[Conclusion] The research provided the theory basis for the comprehensive utilization of Lonicera edulis resource. [Objective] The research aimed to find the optimum proportions of pectase, cellulase and papain in the extraction of Lonicera edulis pigment. [Method] The influences of different proportions of three enzymes on the absorbance value of Lonicera edulis pigment extraction solution were studied by the mixture design.The regression model between the enzyme matching ratio and the absorbance value was established, and the interaction effects of three enzymes were studied. Based on the model optimization, the optimum ratio was obtained. were the pectase 46.6%, cellulase enzyme 17.6% and papain 36.0%. [Conclusion] The research provides the theory basis for the comprehensive utilization of Lonicera edulis resource.
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