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目的研究苦参碱对大鼠铜绿假单胞菌(PA)感染肺组织Th1/Th2机制的调节。方法 40只SPF级SD大鼠,随机分为5组,每组8只,分别为正常对照组、铜绿假单胞菌模型组、苦参碱干预组、左氧氟沙星干预组、左氧氟沙星联用苦参碱组。通过大鼠麻醉、支气管插管注入铜绿假单胞菌方法造模,正常对照组用0.9%Na Cl溶液代替铜绿假单胞菌。各组采取相应干预措施。观察大鼠肺组织病理变化,检测肺组织白细胞介素4(IL-4)、干扰素γ(IFN-γ)细胞因子含量,转录因子T-bet、Gata-3的变化;用RT-PCR半定量法检测IL-4 mRNA、IFN-γmRNA以及T-bet mRNA、Gata-3 mRNA的表达。结果苦参碱组、左氧氟沙星组及左氧氟沙星联用苦参碱组肺组织IL-4含量及IL-4 mRNA均较铜绿假单胞菌模型组降低,而IFN-γ水平及IFN-γmRNA较铜绿假单胞菌模型组升高(P<0.05);苦参碱组、左氧氟沙星组及左氧氟沙星联用苦参碱组中肺组织转录因子Gata-3水平和Gata-3 mRNA表达较铜绿假单胞菌模型组降低(P<0.05),而转录因子T-bet水平和T-bet mRNA表达较铜绿假单胞菌模型组升高(P<0.05)。结论苦参碱能调节大鼠铜绿假单胞菌肺部转录因子T-bet和Gata-3表达,诱导大鼠机体由Th2型向Th1型分化,促进IFN-γ炎症因子合成、抑制IL-4合成,从而减轻肺部组织感染损伤。
Objective To investigate the regulation of matrine on the mechanism of Th1 / Th2 in lung tissue of Pseudomonas aeruginosa (PA) infected rats. Methods Forty Sprague-Dawley (SD) SD rats were randomly divided into five groups (n = 8): normal control group, Pseudomonas aeruginosa model group, matrine intervention group, levofloxacin intervention group, levofloxacin combined with matrine group. Anesthesia in rats, bronchial intubation Pseudomonas aeruginosa injection method, the normal control group with 0.9% NaCl solution instead of Pseudomonas aeruginosa. Each group to take appropriate interventions. The pathological changes in the lung tissue of rats were observed. The levels of IL-4, IFN-γ, cytokine T-bet and Gata-3 were detected by RT-PCR The expression of IL-4 mRNA, IFN-γmRNA, T-bet mRNA and Gata-3 mRNA were detected by quantitative method. Results Compared with Pseudomonas aeruginosa model group, the levels of IL-4 and IL-4 mRNA in lung tissue of matrine group, levofloxacin group and levofloxacin plus matrine group were lower than those of the model group, while the levels of IFN-γ and IFN- (P <0.05). The levels of Gata-3 mRNA and Gata-3 mRNA in lung tissue of matrine group, levofloxacin group and levofloxacin plus matrine group were significantly higher than those of Pseudomonas aeruginosa model (P <0.05). The levels of T-bet and T-bet mRNA in the model group were significantly higher than those in the Pseudomonas aeruginosa model group (P <0.05). Conclusion Matrine can regulate the expression of T-bet and Gata-3 in lung of Pseudomonas aeruginosa, induce the differentiation from Th2 type to Th1 type, promote the synthesis of IFN-γ and inhibit the expression of IL-4 Synthesis, thereby reducing lung tissue infection damage.