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以新疆雪莲快繁无菌苗为试材,研究了新疆雪莲高效植株再生的条件。结果表明:长势健壮的无菌苗叶片切段可以通过2种途径高效再生不定芽;直接器官再生途径:长3~5mm的叶切段在MS+NAA 0.5mg/L+6-BA 2.0mg/L的培养基上先后暗诱导3周和光诱导4周,分化率达(73.3±3.8)%,平均出芽量(5.0±0.3)个芽/块;间接器官再生途径:叶切段在MS+NAA0.2mg/L+TDZ 0.5mg/L培养基上暗诱导3周,愈伤组织诱导率100%,愈伤组织在MS+NAA 0.5mg/L+6-BA 2.0mg/L的培养基上光诱导4周,分化率达(88.9±5.9)%,平均(5.5±0.4)个芽/块;不定芽在MS+NAA 0.05mg/L+6-BA 0.3mg/L培养基中壮苗和增殖培养后,在MS+IBA 0.5mg/L+0.1%活性碳的培养基中生根率达100%;2种植株再生途径均可用于新疆雪莲的诱变育种和转基因育种研究。
Xinjiang lotus seedlings were used as test materials to study the regeneration conditions of Xinjiang snow lotus plants. The results showed that adventitious buds could be regenerated efficiently by two ways in the healthy sterile seedling cuttings. The direct organ regeneration pathway: cuttings with 3 ~ 5mm in length were cultured in the medium of MS + NAA 0.5mg / L + 6-BA 2.0mg / L medium, the rate of differentiation was (73.3 ± 3.8)%, and the average amount of budding was (5.0 ± 0.3) buds per piece. Indirect organ regeneration pathway: Leaf segments cut at MS + NAA0 2mg / L + TDZ 0.5mg / L medium for 3 weeks, the callus induction rate was 100%, and the callus was light on the medium of MS + NAA 0.5mg / L + 6-BA 2.0mg / L After 4 weeks of induction, the rate of differentiation was (88.9 ± 5.9)%, with an average of (5.5 ± 0.4) buds per piece. Adventitious buds grew strongly in medium supplemented with MS + NAA 0.05mg / L and 6-BA 0.3mg / After culturing, the rooting rate reached 100% in MS + IBA 0.5mg / L + 0.1% activated carbon medium. Both regeneration pathways could be used for mutagenesis and transgenic breeding of Xinjiang Xuelian.