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利用化学测定法分析高温、单氰胺和TDZ 3种破眠处理对“曙光”油桃休眠花芽H2O2代谢的主要影响,利用非损伤微测技术检测H2O2对休眠芽Ca2+转运的影响,研究H2O2在芽休眠解除过程中的调控作用.结果表明:在深休眠时期,高温和单氰胺处理均能诱导芽内H2O2含量升高和过氧化氢酶(CAT)活性降低,并具有显著的破眠作用;TDZ对H2O2含量及CAT、过氧化物酶(POD)活性影响不大,破眠效果较差.休眠花芽原基组织钙通道活跃,对外源Ca2+呈吸收状态.外源H2O2可诱导休眠花芽原基组织Ca2+转运发生变化,低浓度H2O2降低Ca2+吸收速率,高浓度H2O2使组织对Ca2+的转运由吸收转变为释放.这表明休眠芽内H2O2信号和Ca2+信号相关联,通过诱导H2O2积累调控Ca2+信号可能在高温和单氰胺打破休眠的信号转导过程中起重要作用.
Chemical analysis was used to analyze the main effects of high temperature, cyanamide and TDZ treatments on H2O2 metabolism in Dormant buds of ’Shuguang’ nectarines. The effect of H2O2 on the Ca2 + transport of dormant buds was examined by non-invasive micro- H2O2 in Bud dormancy release.The results showed that high H2O2 and CAT activity could be induced by high temperature and cyanamide treatment in deep dormancy stage with significant decrease of H2O2 content The effect of TDZ on H2O2 content, CAT and peroxidase (POD) activity was insignificant, and the hypnotic effect was poor.The active calcium channel in dormant bud primordial tissue was active and the exogenous Ca2 + was absorbed.The exogenous H2O2 could induce dormancy Ca2 + transport in flower bud primordial tissue changed, low concentration of H2O2 reduced the rate of Ca2 + uptake, and high concentration of H2O2 changed Ca2 + transport from tissues to release.This indicated that the correlation between H2O2 signal and Ca2 + signal in dormant buds was induced by inducing H2O2 accumulation Ca2 + signaling may play an important role at high temperatures and with cyanamide breaking the dormant signal transduction.