INDUCTION OF SOMATIC EMBRYOS AND REGENERATION OF PLANTLETS FROM RICE ROOTS

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The number of somatic embryos and regenerated plantlets were directly induced from irra-diated rice roots of the variety Taipei 309(Oryza sativa L.).For the induction of somatic em-bryos,the suitable age of roots was no more than 7 days after seed culture on the medium and thesections near tip were more vigorous than those near base of a root;the number of somatic em-bryos induced by the media with 1.11mg/L2,4-D plus 0.47mg/L NAA and 2.21mg/L 2,4-Dplus 1.86mg/L NAA was much higher than that induced by 1.11mg/L 2,4-D alone;10~20 Gyand 40 Gy of γ irradiation were the most favourable for increasing somatic embryo number onthe media of 2.21mg/L 2,4-D plus 1.86mg/L NAA and 1.11mg/L 2,4-D plus 0.47mg/L NAAor 1.11mg/L 2,4-D alone respectively.The highest number of regenerated plantlets was fromthat cultured on the medium of 1.11mg/L 2,4-D plus 0.47mg/L NAA and irradiated with 30~40 Gy of γ rays. The number of somatic embryos and regenerated plantlets were directly induced from irra-diated rice roots of the variety Taipei 309 (Oryza sativa L.). For the induction of somatic em-bryos, the suitable age of roots was no more than 7 days after seed culture on the medium and thesections near tip were more vigorous than those near base of a root; the number of somatic em-bryos induced by the media with 1.11 mg / L2,4-D plus 0.47 mg / L NAA and 2.21 mg / L 2,4-Dplus 1.86 mg / L NAA was much higher than that induced by 1.11 mg / L 2,4-D alone; 10-20 Gy and 40 Gy of γ irradiation were the most favourable for increasing somatic embryo number on the media of 2.21 mg / L 2,4-D plus 1.86 mg / L NAA and 1.11 mg / L 2,4-D plus 0.47 mg / L NAAor 1.11 mg / L 2,4-D alone each.The highest number of regenerated plantlets was fromthat cultured on the medium of 1.11 mg / L 2,4-D plus 0.47 mg / L NAA and irradiated with 30-40 Gy of γ rays.
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