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目的:研究三黄汤、黄连解毒汤和五味消毒饮对超广谱β-内酰胺酶活性及表达的抑制作用以探讨三者对耐药性大肠杆菌的逆转机制。方法:用3种中药方剂培养耐药性大肠杆菌,分析比较逆转前后的超广谱β-内酰胺酶活性和含量。结果:3种中药方剂能够抑制超广谱β-内酰胺酶的活性及表达,与未经任何逆转作用的大肠杆菌阴性对照有显著性差异(P<0.05)。结论:三黄汤、黄连解毒汤和五味消毒饮抑制超广谱β-内酰胺酶的活性和表达是其降低细菌耐药性的机制之一。
Objective: To study the inhibitory effect of Sanhuang Decoction, Huanglian Jiedu Decoction and Wuwei Disinfectant on the activity and expression of extended-spectrum β-lactamases to investigate the mechanism of the reversal of the resistance to Escherichia coli. Methods: Three kinds of traditional Chinese medicine prescriptions were used to culture drug-resistant Escherichia coli. The activity and content of extended-spectrum β-lactamase before and after reversion were analyzed and compared. RESULTS: The three Chinese herbal formulas could inhibit the activity and expression of extended-spectrum β-lactamase, and there was a significant difference (P<0.05) from E. coli negative control without any reversal effect. Conclusion: The inhibition of the activity and expression of extended-spectrum β-lactamase by Sanhuang Decoction, Huanglian Jiedu Decoction and Wuwei Disinfecting Drink is one of the mechanisms of the decrease of bacterial resistance.