论文部分内容阅读
为探讨血管紧张素转化酶基因插入/缺失多态性与冠心病的关系,用多聚酶链反应方法检测79例冠心病患者和68例健康人血管紧张素转化酶基因第16内含子中长度为287bP碱基片段的插入/缺入情况、按其存在与否将研究对象分为缺失型纯合子、插入型纯合子和杂合子,同时检测血清血管紧张素转化酶活性。结果发现冠心病组中缺失型基因频率显著高于对照组(P<0.05),缺失型等位基因频率较对照组亦明显增高(P<0.05)。对照组与冠心病组间血清血管紧张素转化酶活性比较无明显差别,但两组内不同基因型之间血管紧张素转化酶活性均存在显著差别,缺失型最高,插入型最低。以上结果提示,血管紧张素转化酶基因插入/缺失多态性与冠心病有关,缺失型可能是冠心病发生的危险因素之一,血管紧张素转化酶基因缺失型者冠心病的发生可能与其较高的血清转化酶水平有关。
In order to investigate the relationship between angiotensin-converting enzyme gene insertion / deletion polymorphisms and coronary heart disease, the length of the 16th intron of angiotensin-converting enzyme gene was detected by polymerase chain reaction in 79 patients with coronary heart disease and 68 healthy people The insertion / deletion of 287bP base fragment was divided into deletion type homozygotes, insertion homozygotes and heterozygotes according to their existence or not, meanwhile, serum angiotensin converting enzyme activity was detected. The results showed that the frequency of deletion gene in CHD group was significantly higher than that in control group (P <0.05), and the deletion allele frequency was also significantly higher than that in control group (P <0.05). There was no significant difference in serum ACE activity between control group and CHD group, but there was significant difference between the two genotypes in ACE activity, the highest deletion type and the lowest insertion type. The above results suggest that angiotensin converting enzyme gene insertion / deletion polymorphisms and coronary heart disease, loss of type may be one of the risk factors for coronary heart disease, angiotensin-converting enzyme gene deletion of coronary heart disease may be associated with its High levels of serum invertase are relevant.