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肉鳍类是脊椎动物的重要类群,分为总鳍鱼类和四足类.其中总鳍鱼类现生的物种包括腔棘鱼和肺鱼.四足动物则包括两栖类、爬行类、鸟类和哺乳类.为了比较肉鳍类irf-1基因的结构特点和系统发育意义,本研究克隆了肺鱼irf-1基因cDNA全序列,并与肉鳍类中其他物种的irf-1基因进行了比对分析.IRF是与自然免疫相关的蛋白质,目前已发现了11个家族成员,其中irf-1和irf-2是最先被发现的2个成员,在天然免疫中起着重要的作用.但有关IRF-1的报道主要集中在哺乳动物,其他类群中鲜有报道.与已报道的irf-1基因一样,肺鱼的irf-1基因可读框的前345核苷酸非常保守,在其C端也含有1个反式激活区(transactivationdomain)和1个IRF结合域2(IAD2)的模体.虽然肺鱼与四足动物最近的共同祖先距今有417个百万年之久,但跨肉鳍类的序列分析显示,IRF-1氨基酸及其基因序列在肉鳍类中都具有较强的保守性和显著的系统发育意义.用IRF-1氨基酸序列所构建的肉鳍类系统发育与已有报道的结果也是一致的.
Fins are important groups of vertebrates divided into total finfish and quadrupeds, of which the species of total finfish include coelacanths and lungfish, and quadrupeds include amphibians, reptiles, birds In order to compare the structural characteristics and phylogenetic significance of the irf-1 gene in fins, we cloned the full-length cDNA of irf-1 gene from lungfishes and compared the irf-1 gene with other species in fins IRF is a protein related to innate immunity. So far, 11 family members have been found. Among them, irf-1 and irf-2 are the first two members to be found and play an important role in innate immunity However, the report about IRF-1 mainly focused on mammals, but rarely reported in other taxa.As with the reported irf-1 gene, the first 345 nucleotides of the irf-1 gene of lungfish are very conserved, It also contains a motif of one transactivation domain and one IRF binding domain 2 (IAD2) at its C-terminus. Although the common ancestor of the lungfish and quadrupeds lasts 417 million years ago , But the sequence analysis of meat fins shows that IRF-1 amino acid and its gene sequences are highly conserved in meat fins Sexual and significant phylogenetic significance.The phylogenetic relationship of fins with IRF-1 amino acid sequence was also consistent with previous reports.