论文部分内容阅读
目的分析广西沿海地区妇女宫颈病变感染人乳头瘤病毒(HPV)的状况和分布规律;探讨HPV感染与宫颈病变发生的关系。方法将164例临床宫颈有病变、HPV-DNA分型检测阳性患者,按病理检查结果分为3组:慢性炎症组(n=22)、宫颈上皮内瘤变(CINⅠ、Ⅱ、Ⅲ)组(n=73)和宫颈癌组(n=69)。采集患者宫颈脱落细胞样本,用人乳头瘤病毒核酸扩增分型检测方法作HPV基因分型检测,分析HPV感染状况及HPV基因型在各组疾病中的分布。结果 164例HPV阳性标本中,21种亚型有20种亚型被检测到,未被检出是低危型HPV42型;HPV单一感染者117例(71.3%),多重感染47例(28.7%)。慢性炎症组的阳性率13.4%,常见HPV基因型为16、52、58、18、33、53;宫颈上皮内瘤变组阳性率为44.5%,常见HPV基因型为16、18、33、52、58、31;宫颈癌组阳性率为42.1%,常见HPV基因型为16、18、33、31、58、52。结论广西沿海地区妇女宫颈病变以HPV16、18型感染为主,其次是33、52、58、31型。因此,HPV-DNA分型检测对宫颈癌高危人群的筛查、临床诊断、预后判断及疫苗的研制提供重要的理论依据。
Objective To analyze the status and distribution of human papillomavirus (HPV) infection in women with cervical lesions in the coastal areas of Guangxi and to explore the relationship between HPV infection and cervical lesions. Methods One hundred and sixty-four patients with clinical cervical lesions and positive HPV-DNA typing were divided into three groups according to the results of pathological examination: chronic inflammation group (n = 22), cervical intraepithelial neoplasia (CINⅠ, Ⅱ, Ⅲ) n = 73) and cervical cancer (n = 69). The samples of cervical exfoliated cells were collected and detected by HPV genotyping using human papillomavirus nucleic acid amplification typing method to analyze the distribution of HPV infection and HPV genotype in each group of diseases. Results Totally 20 subtypes of 21 HPV subtypes were detected in 164 HPV-positive samples, which were undetected as low-risk HPV type 42; 117 (71.3%) were single-HPV infections and 47 (28.7% ). The positive rate of chronic inflammation group was 13.4%. The common HPV genotypes were 16,52,58,18,33,53. The positive rate of cervical intraepithelial neoplasia group was 44.5%. The common HPV genotypes were 16,18,33,52 , 58,31; The positive rate of cervical cancer was 42.1%, the common HPV genotypes were 16,18,33,31,58,52. Conclusions The cervical lesions of women in coastal areas of Guangxi are mainly HPV16 and HPV18, followed by 33, 52, 58 and 31 cases. Therefore, HPV-DNA typing detection of high risk population of cervical cancer screening, clinical diagnosis, prognosis and vaccine development provides an important theoretical basis.