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目的研究乳酸杆菌对载脂蛋白E(apoE)基因缺陷小鼠动脉粥样斑块形成的影响,探讨乳酸杆菌抗动脉粥样硬化作用机制。方法将apoE基因缺陷(ApoE-/-)小鼠随机分为4组:对照组(A组)、乳酸杆菌108组(B组)、乳酸杆菌1010组(C组)、乳酸杆菌1012(D组)。A组喂养正常小鼠饲料(AIN-93),B、C、D组在正常饲料基础上添加108,1010,1012cfu/mL乳酸杆菌喂养16周,检测动脉粥样斑块面积。结果乳酸杆菌1012,1010,108cfu/mL组的主动脉窦动脉粥样斑块面积分别为13.98%,17.36%,21.54%,比对照组的30.51%分别低54.18%,43.10%,29.40%,差异有统计学意义(P<0.05);随着乳酸杆菌浓度增加,主动脉窦动脉粥样斑块面积减少,但乳酸杆菌各组之间动脉粥样斑块面积差异无统计学意义。结论乳酸杆菌可以抑制ApoE-/-小鼠动脉粥样斑块形成,具有抗动脉粥样硬化作用。
Objective To investigate the effect of Lactobacillus on the formation of atherosclerotic plaque in apolipoprotein E (apoE) -deficient mice and to explore the anti-atherosclerotic mechanism of Lactobacillus. Methods ApoE - deficient (ApoE - / -) mice were randomly divided into 4 groups: control group (group A), lactobacillus group 108 (group B), lactobacillus group 1010 (group C), lactobacillus group 1012 ). Group A was fed with normal mouse diet (AIN-93), and groups B, C and D were fed with lactobacilli 108,1010,1012 cfu / mL for 16 weeks on the basis of normal diet to detect the area of atherosclerotic plaque. Results The area of aortic atherosclerosis in 1012,1010,108cfu / mL Lactobacillus groups were 13.98%, 17.36% and 21.54%, respectively, which were 54.18%, 43.10% and 29.40% lower than that of the control group (P <0.05). With the increase of Lactobacillus concentration, the area of atherosclerotic plaque in aortic sinus decreased, but there was no significant difference in the area of atherosclerotic plaque in each group of Lactobacillus. Conclusion Lactobacillus can inhibit ApoE - / - mice atherosclerotic plaque formation, with anti-atherosclerotic effect.