论文部分内容阅读
目的研究肠道细菌移位在华支睾吸虫病致病机制中的作用。方法建立华支睾吸虫感染大鼠模型。分别在造模后48 h(后尾蚴期)、18 d(童虫期)和35 d(成虫期),取肝、肺、淋巴结和血液组织,采用平板培养法进行细菌移位的检测;采用鲎三肽基质染色定量法检测血浆内毒素含量。结果感染18 d后,实验组肠道细菌移位率开始增高,至感染35 d时,细菌移位率为70%,明显高于对照组的10%,差异有统计学意义(P=0.023<0.05);感染鼠以童虫期、成虫期细菌移位现象明显,总移位率为65%,与对照组10%比较,差异有统计学意义(u=3.59,P<0.01),且在肝、肺、淋巴结和血液组织中,移位发生率分别为60%、15%、25%和10%,以肝脏部位最高;造模后18 d血浆内毒素水平明显增高,造模后35 d血浆LPS水平略有下降,但仍明显高于对照组,差异有统计学意义(t=7.612,P<0.01)。结论华支睾吸虫感染可引发宿主肠道菌群移位,以肝组织多发,从而参与致病机制。
Objective To study the role of intestinal bacterial translocation in the pathogenesis of Clonorchiasis sinensis. Methods The rat model of Clonorchis sinensis infection was established. Bacterial translocation was detected by plate culture method at 48 h (post-cercaria stage), 18 d (sphincter stage) and 35 d (adult stage), respectively.鲎 Tripeptide matrix staining quantitative detection of plasma endotoxin content. Results After 18 days of infection, the bacterial translocation rate of intestinal bacteria began to increase in the experimental group. The bacterial translocation rate was 70% at 35 days after infection, which was significantly higher than that of the control group (P = 0.023 < 0.05). The bacterial translocation in the infected mice was significant at the stage of schistosomiasis and adult stages, with a total shift of 65%, which was significantly different from that of the control group (u = 3.59, P <0.01) In the liver, lung, lymph nodes and blood tissues, the incidence of translocation was 60%, 15%, 25% and 10%, respectively, with the highest part of the liver. Plasma endotoxin levels were significantly increased 18 days after modeling, Plasma LPS levels decreased slightly, but still significantly higher than the control group, the difference was statistically significant (t = 7.612, P <0.01). Conclusion Clonorchis sinensis infection can cause host intestinal flora shift, with multiple liver tissues, which involved in pathogenesis.