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作者研究了114例B细胞起源的慢性淋巴细胞白血病(CLL)病人白血病B细胞的核型,并得到53例细胞遗传学随访资料。采用Ficoll-Hypaque梯度离心分离出病人静脉血中的淋巴细胞,用RPMI 1640培养基及10%胎牛血清等进行培养,获得有丝分裂中期的细胞。细胞核型分析采用G或Q分带技术。53例中男性37例,女性16例,年龄48-88岁,病程1月至12年(大多数在5年以下)。24例未经治疗,29例曾用过瘤可宁、强的松、
The authors studied the karyotype of leukemia B cells in 114 patients with chronic lymphocytic leukemia (CLL) of B-cell origin and obtained 53 cases of cytogenetic follow-up data. Lymphocytes in the venous blood of the patient were separated by Ficoll-Hypaque gradient centrifugation and cultured in RPMI 1640 medium and 10% fetal bovine serum to obtain cells in the middle mitosis. The karyotype analysis uses G or Q banding technology. In 53 cases, there were 37 males and 16 females, aged 48-88 years, and the disease duration was from January to 12 years (most of them were less than 5 years). Twenty-four patients had not been treated, and 29 patients had previously used tumor neoplasms, prednisone,