本文研究了用傅里叶变换近红外漫反射光谱法非破坏性测定完整油菜籽中油份、蛋白质和硫甙含量的方法。结果表明１：分析光束直径对测量有明显的影响；直径越大，效果越好；２：该方法对蛋白质和硫甙含量测定效果较好，相关系数分别为０．９４和０．９５，相对误差分别为２．１％和１６．２％，有望应用于品质育种等生产实践中。 In this paper, the method of non-destructive determination of oil, protein and glucosinolate in intact rapeseed by Fourier transform near infrared diffuse reflectance spectroscopy was studied. The results show that 1: the analysis of the beam diameter has a significant effect on the measurement; the larger the diameter, the better the effect; 2: The determination of protein and glucosinolate content is better, the correlation coefficients were 0.94 and 0.95, The errors are 2.1% and 16.2%, respectively, which are expected to be applied to the production practice of quality breeding.