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根表铁膜是水稻重要的养分库和抵抗环境胁迫的天然屏障.然而根表铁膜形成的分子机制并不清楚.本文以日本晴野生型对照及Osfrdl1突变体为材料,研究了OsFRDL1基因在水稻根表铁膜形成中的作用.结果发现,低磷(<0.02 mmol L-1)是水稻根表铁膜形成的重要条件.当处理溶液pH为5.0~6.0,FeSO4浓度为30~50μmol L-1时,根表铁膜含量最大.与通气处理相比,缺氧处理降低水稻根表铁膜含量;且缺氧处理时,Osfrdl1突变体根表铁膜含量低于野生型.实时定量PCR结果表明,缺氧处理显著增加OsFRDL1基因在根系表达,且根系基部的表达强度高于根尖.OsFRDL1::GUS染色结果表明,OsFRDL1::GUS报告基因主要在根系表皮细胞和维管束细胞表达;缺氧处理增加了报告基因在上述位置的表达.缺氧处理时,野生型比Osfrdl突变体根系具有较高的过氧化物酶活性.上述结果表明,缺氧处理诱导OsFRDL1基因的高量表达,使野生型与突变体相比,过氧化物酶活性维持较高的水平,从而使其铁膜生成量高于突变体.
The root iron membrane is an important nutrient pool and a natural barrier against environmental stress in rice.However, the molecular mechanism of the formation of iron on the root surface is not clear.In this paper, the Japanese wild type control and Osfrdl1 mutant were used to study the effect of OsFRDL1 gene on rice The results showed that low phosphorus (<0.02 mmol L-1) was an important condition for the formation of iron on the surface of rice.When the treatment solution pH was 5.0-6.0, the concentration of FeSO4 was 30-50 μmol L- 1, the content of iron in root surface was the largest.Compared with aeration treatment, hypoxia treatment reduced the content of iron in surface root of rice, and the content of iron in root surface of Osfrdl1 mutant was lower than that of wild type in hypoxia treatment.Results of real-time quantitative PCR The results showed that hypoxia treatment significantly increased the expression of OsFRDL1 in roots and the expression of OsFRDL1 at the base of roots.The results of OsFRDL1 :: GUS staining showed that OsFRDL1 :: GUS was mainly expressed in root epidermal cells and vascular bundles. Oxygen treatment increased the expression of the reporter gene in these locations.When hypoxia treatment, the wild type had higher peroxidase activity than the Osfrdl mutant.The above results indicated that hypoxia treatment induced the high expression of OsFRDL1 gene, wild Compared with the mutant peroxidase activity to maintain a high level, so that it is higher than the amount of iron plaque mutants generated.