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目的:了解阳江市地区不孕不育患者解脲支原体(UU)和沙眼衣原体(CT)感染情况,探讨荧光定量PCR(FQ-PCR)应用于诊治不孕不育的必要性。方法:采用FQ-PCR方法检测不孕不育患者宫颈分泌物、尿道拭子标本中的UU-DNA和CT-DNA含量。结果:238例不孕不育患者中有129例感染UU或CT(感染率54.2%),UU和CT的感染率分别为44.5%和17.6%,混合感染率8.0%;UU和CT感染率分别为男性40.6%和25.6%,女性46.7%和13.1%;女性与男性患者UU和CT的基因拷贝数差异无显著性(P>0.05)。结论:UU和CT是引起男女不孕不育的重要原因,UU感染率明显高于CT;FQ-PCR技术应用于诊治UU或CT泌尿生殖道感染引起的不孕不育快捷精确、安全可靠。
Objective: To understand the prevalence of UU and CT infection in infertile patients in Yangjiang city and to explore the necessity of using fluorescence quantitative PCR (FQ-PCR) in the diagnosis and treatment of infertility. Methods: The contents of UU-DNA and CT-DNA in cervical secretions and urethral swab specimens of infertility patients were detected by FQ-PCR. Results: Among 238 infertility patients, 129 were infected with UU or CT (infection rate was 54.2%). The infection rates of UU and CT were 44.5% and 17.6% respectively, and the mixed infection rate was 8.0%. The infection rates of UU and CT were respectively 40.6% and 25.6% for males and 46.7% for females and 13.1% for women. There was no significant difference in gene copy number between UU and CT in female and male patients (P> 0.05). Conclusion: UU and CT are the main causes of infertility in men and women. The infection rate of UU is obviously higher than that of CT. The infertility caused by UU or CT genitourinary tract infection by FQ-PCR technique is fast, accurate, safe and reliable.