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作为一种模式生物,斑马鱼具有其他动物模型所不具备的诸多优势,非常适用于进行人类疾病建模及机制研究。斑马鱼胰腺经历两次发育分化过程,其间伴有多种信号途径的活化参与。成鱼的胰腺结构与其他动物胰腺相似,能分泌包括胰岛素在内的多种激素。斑马鱼胰腺及对胰岛素敏感的肝脏、肌肉等这些与代谢调控相关的主要器官及外周组织,在进化上具有保守性,糖代谢调控机制也与其他哺乳动物相似,这使得斑马鱼非常适用于糖代谢调控研究。除利用STZ诱导胰岛β细胞凋亡造成斑马鱼糖尿病模型外,高糖溶液交替暴露及外源性干预代谢相关信号途径也可以造成斑马鱼血糖升高。成鱼血糖水平、幼鱼组织糖含量、视网膜及视网膜血管形态变化是目前斑马鱼糖尿病模型研究中的常用检测指标。
As a model organism, zebrafish has many advantages that other animal models do not have and is very suitable for modeling and studying human diseases. The zebrafish pancreas undergoes two stages of development and differentiation, accompanied by the activation of multiple signaling pathways. Pancreatic structure of adult fish and other animals similar to the pancreas, can secrete a variety of hormones, including insulin. Zebrafish pancreas and insulin-sensitive liver, muscle and other major organs and peripheral tissues related to metabolism regulation are evolutionarily conservative and the mechanism of regulation of glucose metabolism is also similar to other mammals, which makes zebrafish very suitable for sugar Metabolic regulation research. In addition to the use of STZ-induced pancreatic β-cell apoptosis caused by zebrafish diabetes model, alternating high glucose solution exposure and exogenous intervention metabolic signaling pathways can also cause increased blood glucose in zebrafish. Fish blood glucose levels, juvenile tissue sugar content, retinal and retinal vascular morphological changes in the current zebrafish diabetes model studies commonly used indicators.