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目的:探讨Tiron能否有效对抗过氧化氢(H_2O_2)对离体培养小鼠Corti器内、外毛细胞的损伤。方法:采用新生小鼠Corti器体外培养技术,建立外源性H_2O_2损伤内外毛细胞模型,给予不同浓度的Tiron,观察对H_2O_2损伤的保护作用、结果:H_2O_2浓度大于1.0mmol/L时,基底膜顶回、中回和底回毛细胞损伤程度不同,底回较重,顶回和中回损伤程度在统计学上无差别。在低于0.5mmol/L时毛细胞损伤与位置无关。在培养液中加入Tiron(10 mmol/L)可以明显减轻H_2O_2对毛细胞的损伤程度。当H_2O_2浓度在0.01～1.0 mmol/L之间时,Tiron几乎可以完全抑制毛细胞的损伤和缺失。结论:Tiron对于小鼠离体培养的Corti器具有明显的保护作用,其对抗H_2O_2损伤的机制可能通过清除超氧阴离子O_2~-和螯合铁离子共同发挥作用。
AIM: To investigate whether Tiron can effectively prevent the injury of Corti cells in vitro and in vitro by hydrogen peroxide (H_2O_2). Methods: Newborn mouse Corti instrument was used to establish the model of exogenous H 2 O 2 injury in vitro and in vivo. Tiron was given to different concentrations of Tiron to observe the protective effect on H 2 O 2 injury. Results: When H 2 O 2 concentration was above 1.0 mmol / L, Top back, back and bottom hair cells in different degrees of damage, the bottom back heavier, top and middle back injury in the statistical no difference. Hair cell damage at less than 0.5 mmol / L is site-independent. Adding Tiron (10 mmol / L) into the culture medium can obviously reduce the damage of H 2 O 2 to hair cells. When H_2O_2 was between 0.01 mmol / L and 1.0 mmol / L, Tiron almost completely inhibited hair cell damage and loss. CONCLUSION: Tiron has obvious protective effect on Corti’s in vitro, and its mechanism against H 2 O 2 may play its role by removing superoxide anion O_2 ~ - and chelating iron ions.