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目的:探讨抑制Wnt/β-catenin信号通路后食管癌细胞对化疗药物顺铂敏感性的变化。方法:双链SiRNA转染干扰β-catenin基因表达;半定量RT-PCR和Western blot检测基因沉默效果;MTT细胞毒实验检测细胞的生长抑制率。结果:合成的Wnt/β-catenin SiRNA可以显著下调β-cateninmRNA和蛋白的表达水平,对内参对照基因GAPDH没有影响;对照组,SiRNA干扰组,顺铂处理组,SiRNA干扰+顺铂处理组的细胞存活率分别为(100±6.8)%、(87.3±5.2)%、(66.4±4.4)%和(41.8±3.64)%。顺铂处理组与SiRNA干扰+顺铂处理组比较细胞的存活率降低增加了大约30%。结论:干扰β-catenin的表达能显著增强食管癌细胞对化疗药物顺铂的敏感性,为减少化疗药物顺铂用量和其耐药的克服带来了曙光,也为食管癌等恶性肿瘤的化学治疗开辟新途径提供了理论依据。
OBJECTIVE: To investigate the changes of chemosensitivity to cisplatin in esophageal cancer cells after inhibition of Wnt / β-catenin signaling pathway. Methods: The expression of β-catenin gene was interfered by double-stranded SiRNA transfection. The effect of gene silencing was detected by semi-quantitative RT-PCR and Western blot. The growth inhibition rate was detected by MTT cytotoxicity assay. Results: The synthetic Wnt / β-catenin SiRNA could significantly down-regulate the expression of β-catenin mRNA and protein, and had no effect on the GAPDH control gene. In the control group, SiRNA interference group, cisplatin treatment group, SiRNA interference + cisplatin treatment group Cell survival rates were (100 ± 6.8)%, (87.3 ± 5.2)%, (66.4 ± 4.4)% and (41.8 ± 3.64)%, respectively. Cisplatin treatment group and SiRNA interference + cisplatin treatment group decreased cell viability decreased by about 30%. Conclusion: The interference of β-catenin expression can significantly enhance the sensitivity of esophageal cancer cells to chemotherapeutic agents cisplatin, to bring about the dawn to reduce the dosage of chemotherapeutic agents cisplatin and its resistance, but also for the chemotherapy of esophageal cancer and other malignant tumors Therapeutic open up new ways to provide a theoretical basis.