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应用“311-B”回归最优设计研究抗坏血酸、亚硫酸钠、柠檬酸对东方杉愈伤组织褐化率的影响。得出培养14d时的回归方程为Y2=0.688+0.586X1-0.211X2+0.523X3+0.481X21+0.612X22+1.312X23+0.633X1X2+0.075X1X3-0.978X2X3,其Ryy=0.9822**,愈伤组织褐化率与试剂处理呈极显著相关。结果表明:(1)3种试剂单独使用时对愈伤组织褐化抑制效果与培养时期有关,在培养初期抑制褐化效果为柠檬酸>抗坏血酸>亚硫酸钠。随培养时间延长到14d时,抗坏血酸与柠檬酸的效果逐渐减弱或消失,而亚硫酸钠的作用最为明显,褐化率降到21%,使用浓度为0.438g·L-1。(2)在培养期间3种试剂配合使用对愈伤组织褐化抑制有明显的交互作用,培养14d时,褐化率降到8%,抗坏血酸、亚硫酸钠、柠檬酸三者配合使用最佳浓度分别为0.25、0.25和5g·L-1。各种试剂对东方杉愈伤组织褐化抑制效应与其化学性质有关。
The optimal design of “311-B” regression was used to study the effects of ascorbic acid, sodium sulfite and citric acid on the browning rate of Cunninghamia. The results showed that the regression equation of 14d was Y2 = 0.688 + 0.586X1-0.211X2 + 0.523X3 + 0.481X21 + 0.612X22 + 1.312X23 + 0.633X1X2 + 0.075X1X3-0.978X2X3 with Ryy = 0.9822 **, callus Browning rate and reagent handling were extremely significant correlation. The results showed that: (1) The inhibition of browning of callus by 3 kinds of reagents was related to the culture period, while the inhibition of browning was citric acid> ascorbic acid> sodium sulfite at the beginning of culture. The effect of ascorbic acid and citric acid gradually diminished or disappeared with the incubation time prolonged to 14 days, while the effect of sodium sulfite was the most obvious. The browning rate was reduced to 21% with a concentration of 0.438 g · L -1. (2) During the cultivation, the interaction of 3 kinds of reagents with the browning inhibition of callus showed obvious interaction. During the 14th day of culture, the browning rate was reduced to 8%. The optimum concentrations of ascorbic acid, sodium sulfite and citric acid 0.25, 0.25 and 5 g · L -1. Various reagents on the Oriental callus browning inhibition and its chemical properties.