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目的探讨抑癌基因p16、p21和p27的多态性与上皮性卵巢癌易感性的关联。方法选取2007年3月-2011年4月间120例卵巢癌患者作为考察对象,同时选取120例健康女性人群作为对照组。分别采集外周静脉血5ml,提取基因组DNA,分析每个样本的基因型,并做统计学分析。结果两组的p16基因C540G的C/C、C/G和G/G基因型频率分布无显著性差异(P>0.05);两组的p16基因C580T的C/C、C/G和G/G基因型频率分布无显著性差异(P>0.05);两组p21基因的T/T、T/C和C/C基因型频率分布无显著性差异(P>0.05);两组的p27基因的V/V、V/G和G/G基因型频率分布有显著性差异(P<0.05)。结论 p16基因和p21基因型的多态性上皮性卵巢癌的易感性有关;p27基因的V/V纯合型可能会增加卵巢癌的发病风险,值得临床关注。
Objective To investigate the association between the polymorphisms of the tumor suppressor genes p16, p21 and p27 and the susceptibility to epithelial ovarian cancer. Methods A total of 120 ovarian cancer patients from March 2007 to April 2011 were selected as study subjects, and 120 healthy female subjects were selected as the control group. Peripheral venous blood were collected 5ml, genomic DNA were extracted, the genotype of each sample was analyzed, and statistical analysis. Results There was no significant difference in the frequencies of C / C, C / G and G / G genotypes between the two groups (P> 0.05). The C / C, C / G and G / (P> 0.05). There was no significant difference in the frequency distribution of T / T, T / C and C / C genotypes between the two groups (P> 0.05). The p27 gene The frequency distribution of V / V, V / G and G / G genotypes was significantly different (P <0.05). Conclusion The p16 gene is associated with the polymorphism of p21 genotype in epithelial ovarian cancer. V / V homozygote of p27 gene may increase the risk of ovarian cancer, which deserves clinical attention.