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目的:研究β2肾上腺素能激动剂福莫特罗(Formoterol)对大鼠的体外骨髓间充质干细胞(MMSC)向成骨细胞分化的影响,进而探讨其作用机制。方法:取SD大鼠的骨髓间充质干细胞,用条件培养液诱导分化后分别加入不同浓度药物,在不同时间点采用RT-PCR法检测细胞分化中Runx2和Osterix的mRNA的表达,采用westernblot法检测细胞中MEK和ERK1/2的磷酸化。结果:在细胞分化早期,加入已知浓度10-7mol/L的Formoterol可抑制成骨样细胞细胞特异性转录因子Runx2mRNA表达;在细胞分化晚期,浓度10-7mol/L的Formoterol也可抑制成骨样细胞OsterixmRNA表达。在加入浓度10-7mol/的Formoterol作用30min后,MEK和ERK1/2的蛋白磷酸化表达均下降。结论:β2受体激动剂可抑制MMSC细胞体外向成骨样细胞的分化,并且可抑制MEK和ERK1/2磷酸化的表达。
AIM: To investigate the effect of Formoterol, a β2-adrenergic agonist, on the differentiation of rat bone marrow mesenchymal stem cells (MMSCs) into osteoblasts in vitro and to explore its possible mechanism. METHODS: Bone marrow-derived mesenchymal stem cells from SD rats were cultured in conditioned media and different concentrations of drug were added respectively. The expression of Runx2 and Osterix mRNA was detected by RT-PCR at different time points. The phosphorylation of MEK and ERK1 / 2 in the cells was examined. Results: In the early stage of cell differentiation, adding Formoterol with known concentration of 10-7mol / L inhibited osteoblast-like cell-specific transcription factor Runx2mRNA expression; Formoterol with concentration of 10-7mol / L also inhibited osteogenic Like cell Osterix mRNA expression. The protein phosphorylation of MEK and ERK1 / 2 decreased after adding 10-7mol / L Formoterol for 30min. CONCLUSION: β2 agonists can inhibit the differentiation of MMSC cells into osteoblast-like cells in vitro and inhibit the phosphorylation of MEK and ERK1 / 2.