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以海南、浙江和江西稻区的细菌性条斑病(以下简称细条病。编者)混合菌株为抗原免疫家兔,获得了效价为9000倍的抗血清。该抗血清特异性强,对常见十余种植物病原细菌基本上无交叉反应;以酶标A蛋白为关键试剂建立的液相酶联法的灵敏度约10~3cfu/ml;样品检测批间和批内的变异系数分别小于24.5％和15.2％。1990～1991年利用该方法检测了海南、浙江、江西及江苏的419份稻种,对除江苏外的363份样品的
The antisera with the potency of 9000 times was obtained by immunizing rabbits with the mixed bacterial strain of Bacterial Leaf Streak (hereinafter referred to as Streptomyces) compiled from Hainan, Zhejiang and Jiangxi Provinces. The antiserum has strong specificity and basically no cross-reaction to common plant pathogens. The sensitivity of the liquid phase enzyme-linked assay with enzyme A protein as the key reagent is about 10-3 cfu / ml. The coefficient of variation within the batch was less than 24.5% and 15.2% respectively. From1991 to 1991, 419 rice varieties from Hainan, Zhejiang, Jiangxi and Jiangsu were detected by this method. Among 363 samples except Jiangsu