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大黄酸的测定，是利用高效液相色谱测定结合大黄酸的含量。由于水解后样品溶液的酸度使分析结果重视性差，故采用加缓冲液控制溶液的酸度，从而提高了分析结果的准确度，并延长了色谱柱的寿命。
Determination of rhein is the use of high performance liquid chromatography to determine the content of Rhein bound. Since the acidity of the sample solution after hydrolysis makes the analysis result less important, the buffer is used to control the acidity of the solution, thereby improving the accuracy of the analysis results and prolonging the life of the column.