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目的探讨MIB-1在子宫颈鳞癌的表达及临床意义。方法应用免疫组化技术检测28例正常子宫颈上皮(NCE),36例子宫颈上皮内瘤变(CIN)和68例子宫颈鳞癌(SCC)组织增殖指数(PI,MIB-1标记)、微血管密度(MVD,CD34标记)和微淋巴管密度(LMVD,VEGFR-3标记)。结果 MIB-1阳性染色定位于肿瘤细胞核。MIB-1标记的PI在NCE、CIN及SCC组分别为96.0±54.6、300.0±157.1和378.7±199.6。从NCE到CIN再到SCC组,PI显著升高(P<0.05)。MIB-1在CIN及SCC组表达均分别与MVD显著正相关(CIN:r=0.442,P=0.007;SCC:r=0.253,P=0.037),而与LMVD无显著相关性(CIN:r=0.099,P=0.567;SCC:r=0.159,P=0.196)。MIB-1在SCC组表达分别与有否盆腔淋巴结转移、脉管浸润、间质浸润深度及生存率有关(P<0.05)。有盆腔淋巴结转移、脉管浸润及突破深肌层间质浸润者,其PI均分别显著高于无盆腔淋巴结转移、无脉管浸润及未突破深肌层间质浸润者(P<0.05)。MIB-1高表达者生存率显著低于低表达者(P=0.0383)。结论 MIB-1可能在子宫颈鳞癌发生和浸润转移过程中发挥重要作用,可能是子宫颈鳞癌患者不良预后的标记物。
Objective To investigate the expression of MIB-1 in cervical squamous cell carcinoma and its clinical significance. Methods 28 cases of normal cervical epithelium (NCE), 36 cases of cervical intraepithelial neoplasia (CIN) and 68 cases of cervical squamous cell carcinoma (SCC) proliferation index (PI, MIB-1) were detected by immunohistochemistry. The microvessel density (MVD, CD34 labeling) and lymphatic vessel density (LMVD, VEGFR-3 labeling). Results MIB-1 positive staining located in the tumor nucleus. The MIB-1 labeled PI was 96.0 ± 54.6, 300.0 ± 157.1 and 378.7 ± 199.6 in the NCE, CIN and SCC groups, respectively. From NCE to CIN to SCC, PI was significantly increased (P <0.05). The expression of MIB-1 in CIN and SCC was significantly and positively correlated with MVD (CIN: r = 0.442, P = 0.007; SCC: r = 0.253, 0.099, P = 0.567; SCC: r = 0.159, P = 0.196). The expression of MIB-1 in SCC group was correlated with pelvic lymph node metastasis, vascular invasion, depth of interstitial infiltration and survival (P <0.05). Patients with pelvic lymph node metastasis, vascular invasion and breakthrough of deep myometrial interstitial infiltration had significantly higher PIs than those without pelvic lymph node metastasis, without vascular infiltration and without infiltration of deep muscular layer (P <0.05). The survival rate of MIB-1-overexpressors was significantly lower than that of MIB-1 overexpressors (P = 0.0383). Conclusion MIB-1 may play an important role in carcinogenesis and invasion and metastasis of cervical squamous cell carcinoma, which may be a marker of poor prognosis in patients with cervical squamous cell carcinoma.