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目的观察通心络胶囊对大脑中动脉闭塞(MCAO)模型大鼠脑缺血后微血管的保护作用。方法清洁级雄性 SD 大鼠200只随机分为5组:假手术组只做颈总和颈内动脉分离和生理盐水灌胃;其余4组制备 MCAO 模型,其中 MK-801组在造模成功后腹腔注射 MK-80I;通心络大、小剂量组均在造模成功后分别给予1.0 g·kg~(-1)·d~(-1)和0.5 g·kg~(-1)·d~(-1)通心络原粉水溶液灌胃;模型组给予等体积生理盐水灌胃。各组动物分别于术后6、12、24、48和72 h 电镜观察皮质微血管超微结构改变、检测缺血脑皮质毛细血管通透性、免疫组化法测定大脑皮质Ⅷ因子和脑微血管周围 IL-6、IL-1β蛋白表达。结果电镜下观察,治疗组均能显著改善缺血后脑皮质微血管超微结构;模型组脑内伊文蓝含量明显增加(P<0.01),通心络大剂量组在脑缺血12 h 能显著降低其含量(P<0.01),并且在24 h 后较小剂量组、MK-801组降低明显(P<0.01);模型组皮质中微血管数急剧减少(P<0.01),治疗组各时间皮质中血管数均明显多于模型组(P<0.01),其中通心络大剂量组的微血管数多于其他治疗组(P<0.01,P<0.05);模型组脑皮质微血管周围 IL-6及 IL-1β表达升高(P<0.01),分别于缺血后24和12 h 达到高峰,各治疗组与模型组相比,IL-6表达明显升高(P<0.01),而 IL-1β表达明显降低(P<0.01),其中通心络大剂量组最为明显(P<0.01)。结论通心络胶囊可从多角度对大脑中动脉闭塞(MCAO)模型大鼠脑缺血微血管损伤进行保护。
Objective To observe the protective effect of Tongxinluo capsule on microvasculature after cerebral ischemia in MCAO model rats. Methods 200 male clean SD rats were randomly divided into 5 groups: the sham group was only separated from the common carotid artery and internal carotid artery and the saline was administered intragastrically; the other 4 groups were prepared MCAO model, in which the MK-801 group was abdominal cavity after successful modeling. Injection of MK-80I; Tongxinluo large and small dose groups were given 1.0 g·kg~(-1)·d~(-1) and 0.5 g·kg~(-1)·d~ respectively after successful modeling. (-1) The Tongxinluo original powder aqueous solution was given to the stomach; the model group was given an equal volume of saline to gavage. The ultrastructural changes of cortical microvessels were observed by electron microscopy at 6, 12, 24, 48, and 72 hours after operation. The capillary permeability of ischemic cerebral cortex was measured by electron microscopy. The cerebral cortex VIII factor and brain microvessels were measured by immunohistochemistry. IL-6, IL-1β protein expression. RESULTS: Under the electron microscope, the treatment group all significantly improved the ultrastructure of the microvessels in the cerebral cortex after ischemia; the content of Evan Blue in the brain of the model group increased significantly (P<0.01), and the high-dose Tongxinluo group significantly reduced the cerebral ischemia at 12 h. The content (P<0.01) decreased significantly after 24 h in the small dose group and MK-801 group (P<0.01); the number of microvessels in the model group decreased sharply (P<0.01), and the time in the cortex of the treatment group The number of blood vessels was significantly more than that of the model group (P<0.01), and the number of microvessels in the high-dose Tongxinluo group was more than other treatment groups (P<0.01, P<0.05); IL-6 and IL around the cerebral cortex microvessels in the model group. The expression of -1β was elevated (P<0.01), peaked at 24 and 12 h after ischemia, and the expression of IL-6 was significantly increased (P<0.01) and IL-1β was expressed in each treatment group compared with the model group. Significantly lower (P <0.01), Tongxinluo large dose group is the most obvious (P <0.01). Conclusion Tongxinluo Capsule can protect cerebral ischemia microvascular injury in middle cerebral artery occlusion (MCAO) model rats from multiple angles.