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目的:观察参麦注射液对猪离体冠状动脉环的舒张作用,并探讨其作用机制。方法:采用离体血管张力的实验方法,观察0.1,0.5,1,5,10,50 mL.L-1的参麦注射液对KCl(30 mmol.L-1)诱发的猪离体冠脉环收缩的影响。观察内皮、Na+/Ca2+交换体阻断剂KB-R7943(1×10-6 mol.L-1)、KV通道阻断剂四胺基吡啶(4-AP,1×10-3 mol.L-1)、KATP通道阻断剂格列苯脲(Gli,1×10-5 mol.L-1)、KCa通道阻断剂四乙胺(TEA,1×10-2 mol.L-1)、KiR通道阻断剂氯化钡(BaCl2,1×10-3mol.L-1)对参麦注射液作用的影响。结果:参麦注射液对KCl(30 mmol.L-1)预收缩的猪离体冠脉环产生浓度依赖性的舒张作用,去内皮,KB-R7943,BaCl2及Gli预处理的血管环对参麦注射液的舒张反应与未经处理时比较无显著性差异。4-AP及TEA可显著减弱参麦注射液对血管环的舒张作用(P<0.05)。结论:参麦注射液对KCl预收缩的猪离体冠脉环具有浓度依赖性的舒张作用,其舒张反应无内皮依赖性,与Na+/Ca2+交换体无关,可能与KCa通道及KV通道有关。
Objective: To observe the effect of Shenmai injection on isolated porcine coronary rings and its mechanism. Methods: The effects of Shenmai injection 0.1,1.5,1,5,10,50 mL.L-1 on KCl (30 mmol.L-1) induced porcine isolated coronary artery Ring contraction effect. The effects of K (superscript +) channel blocker, 4-AP (1 × 10-3 mol·L-1), Na + / Ca2 + exchanger blocker KB-R7943 (1 × 10-6 mol·L- 1), KATP channel blocker glibenclamide (Gli, 1 × 10-5 mol·L-1), KCa channel blocker tetraethylammonium (TEA, 1 × 10-2 mol·L-1) Effect of KiR channel blocker barium chloride (BaCl2, 1 × 10-3mol.L-1) on the effect of Shenmai injection. Results: Shenmai injection had a concentration-dependent relaxation effect on porcine isolated coronary artery rings precontracted with KCl (30 mmol.L-1). The vascular rings treated with endothelium, KB-R7943, BaCl2 and Gli preconditioned There was no significant difference in the diastolic response of Mai injection compared with that of the untreated group. 4-AP and TEA significantly attenuated the relaxation effect of Shenmai injection on vascular rings (P <0.05). Conclusion: Shenmai injection has a concentration-dependent relaxation effect on KC1 precontracted porcine coronary coronary artery rings. The diastolic response is independent of Na + / Ca2 + exchanger and may be related to KCa channel and KV channel.