用采自中国河南省 (HN)、河北省 (HB)及瑞典 (SW )HCV感染者的血清标本 ,研究不同地区HCV感染病人同一基因型 (1b)膜区 (E2 )基因变化。利用RT—PCR对HCV膜区基因扩增并测定序列。结果显示不同地区分离的同一基因型HCV膜区序列各异 ,相同地域基因同源性较高。变化最大的区域是HVR区 ,核苷酸变化导致了氨基酸序列改变。提示区域性变化可能是HCV变异的一个重要方面 Serum samples collected from HCV infected persons in Henan Province (HN), Hebei Province (Hebei Province) and Sweden (SW) were used to study the changes of E2 gene in the same genotype (1b) in patients with HCV infection in different areas. The HCV membrane region was amplified by RT-PCR and sequenced. The results showed that the HCV genomic regions of the same genotype isolated in different regions had different sequences and the homology of the same region was high. The area of ​​greatest change is the HVR region, and nucleotide changes result in amino acid sequence changes. Suggesting that regional changes may be an important aspect of HCV variation