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目的探讨过氧化物酶体增生物激活受体γ(PPARγ)在大鼠出生前后心肌组织发育中的表达规律。方法利用反转录-PCR和免疫蛋白印迹技术,检测18～20 d胎鼠组(胎儿期)、出生5～6 d(相当于人新生儿期)、出生10～12 d(相当于人幼儿期)和出生18～20 d新生大鼠(相当于人7～13岁)(每组各6只)心肌组织PPARγ mRNA及其蛋白的表达,并利用图像分析系统对其表达结果进行定量分析。结果胚胎18～20 d胎鼠心肌组织中PPARγ mRNA呈强阳性表达,出生后各组逐渐减弱;从胚胎18 d至出生18～20 d大鼠心肌组织中PPARγ蛋白的表达强度逐渐减弱,最终呈稳定表达。结论 PPARγ在大鼠心肌组织中的表达规律是从胎心开始,随着心脏的发育,心肌细胞中的PPARγ表达逐渐减少,提示PPARγ对心肌细胞的分化具有重要的调节作用。
Objective To investigate the expression of peroxisome proliferator - activated receptor γ (PPARγ) in myocardial tissue before and after birth. Methods Reverse transcription-PCR and Western blotting were used to detect fetuses aged 18-20 days (fetal period), born 5-6 days (equivalent to human neonatal period) and born 10-12 days (equivalent to human children (Corresponding to 7 ~ 13 years old) (6 in each group), and the expression of PPARγ mRNA and protein were detected by image analysis system. Results The expression of PPARγ mRNA in fetal heart tissue of embryos from 18 to 20 days was strongly positive, and gradually decreased after birth. From 18 d to 18 to 20 d, the PPARγ protein expression gradually decreased in the myocardium, and finally decreased Stable expression. Conclusions The expression of PPARγ in rat myocardium starts from the fetal heart. With the development of the heart, the expression of PPARγ gradually decreases, suggesting that PPARγ plays an important regulatory role in the differentiation of cardiomyocytes.