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目的对喉部MSCT和组织切片图像及其三维重建的比较研究。方法 30例(21男,9女)结构完整的喉标本,全喉连续大切片,HE染色,专业微距照相系统拍照,专业图像分析;30名(12男,18女)健康志愿者经64排高分辨率薄层MSCT扫描,得到喉部CT图像。在用3D-Doctor软件进行MSCT三维重建。测量组织切片和MSCT三维重建喉甲状软骨、环状软骨和6个切面会厌前间隙和声门旁间隙的面积,进行两组独立样本的t检验。结果 MSCT图像中甲状软骨、会厌软骨和环状软骨能清晰显示,但杓状软骨显示不全。会厌前间隙和声门旁间隙内容结构无法显示,而组织切片清晰显示间隙内容。组织切片和MSCT甲状软骨、环状软骨的测量结果无显著性差异(P>0.05)。除了甲状软骨声带附着处至上、下切迹距离等四项数据无性别差异(P>0.05)以外,其他数据均有性别差异(P<0.05)。MSCT切割平面与组织切片会厌前间隙和声门旁间隙面积的测量结果无显著性差异(P>0.05)。结论 MSCT对超过其分辨率的细微结构显示欠佳,其三维重建的细节效果不如组织切片完整清晰。组织切片能对MSCT起到良好的补充作用,使得MSCT及其三维重建作为临床医疗诊断和影像学检查的辅助工具,更适合于临床应用。
Objective To compare the throat MSCT and tissue section images and their 3D reconstruction. Methods Thirty (21 males and 9 females) throat specimens with complete throat, throat specimens of full throat, HE staining, professional macro photography system and professional image analysis were included in the study. Thirty (12 males and 18 females) healthy volunteers High-resolution thin-layer MSCT scan, throat CT images. MSCT 3D reconstruction with 3D-Doctor software. Tissue sections and MSCT three-dimensional reconstruction of laryngeal thyroid cartilage, cricoid cartilage, and six sections of anterior metacarpophalangeal and supraglottic space area were measured by t test for two independent sets of samples. Results Thyroid cartilage, epiglottic cartilage and cricoid cartilage were clearly shown in the MSCT images, but the arytenoid cartilage was incomplete. Epiglottic space and glottic gap structure of the contents of the gap can not be displayed, and tissue sections clearly show the gap content. Tissue sections and MSCT thyroid cartilage, annular cartilage measurements showed no significant difference (P> 0.05). There was no significant difference (P> 0.05) in the other data except for the four data of upper cartilage attached to the upper and lower notch distance (P> 0.05). There were no significant differences in the measurements of the anterior epiglottic space and glottic gap between the MSCT cut plane and the tissue section (P> 0.05). Conclusion MSCT shows subtle structure beyond its resolution, and the details of 3D reconstruction are not as complete as the histological sections. Tissue sections can be a good complement to MSCT, making MSCT and its 3D reconstruction as an auxiliary tool for clinical diagnosis and imaging examination, which is more suitable for clinical application.