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三、急性麻痹病(Acuie paralysis) 该病由急性麻痹病毒(Aucle bee-paraeysis virus)引起,其症状与慢性麻痹病相似,只是病原不同。病毒颗粒为直径25—30μm等轴颗粒。RNA型,用磷钨酸负性染色观察为空心颗粒,沉降后可分为两部分,沉降系数分别为160S,80S,在氯化铯中浮密度为1.34克/毫升,E260/E280之比等于1.64。它们核酸碱基之比(A∶C∶G∶u)=30.3∶20.5∶18.8∶30.4。一只急性麻痹病蜜蜂可以提取10~(12)病毒颗粒注入血淋巴中10~2的病毒可引起发病,饲喂需要10~(11)颗粒。这种病毒在幼虫体内不
Third, acute paralysis (Acuie paralysis) The disease is caused by the acute paralytic virus (Aucle bee-paraeysis virus), its symptoms and chronic paralysis similar to, but different pathogens. Virus particles are 25-30 μm in diameter. RNA-type, hollow microspheres were observed by negative staining with phosphotungstic acid. The sedimentation was divided into two parts and the sedimentation coefficients were 160S and 80S respectively. The floating density in cesium chloride was 1.34 g / ml and the ratio of E260 / E280 was equal to 1.64. Their nucleic acid base ratio (A: C: G: u) = 30.3: 20.5: 18.8: 30.4. An acute paralysis bee can extract 10 ~ (12) virus particles injected into the hemolymph virus 10 ~ 2 can cause disease, feeding 10 ~ (11) particles. This virus is not in larvae