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目的研究宾赛克嗪(新型抗毒剂)与阿托品对肠粘膜上皮细胞形态学改变的比较影响,确定有机磷农药中毒临床治疗剂量下的药物选择依据。方法 42只健康雄性昆明小鼠,随机分为生理盐水组、宾赛克嗪组和阿托品组(药物对照组),后两组又分别分为3个剂量组(2、6和18 mg/kg),于0、2、4、8、10、12、24、48、72、96、120和168 h腹腔注射,然后处死。取盲肠末端的整个结肠和回肠段,在光学显微镜下,观察结肠与回肠部位的肠上皮细胞形态学变化。结果 2 mg/kg剂量组(宾赛克嗪与阿托品):回肠绒毛较为平坦,结肠肌层增厚有少量炎性渗出;6 mg/kg剂量组:回肠与结肠表现为少量嗜酸性粒细胞浸润,粘膜下层水肿,肌层增厚;18 mg/kg剂量组:回肠与结肠表现为水肿渗出严重,淋巴组织大量增生,炎症细胞浸润,其中回肠部位肠腺有脓肿,少量上皮细胞脱落坏死等。结论两种药物均对肠粘膜有损伤作用,剂量越大,对肠粘膜的损伤越严重。但是,在同一临床治疗剂量下,宾赛克嗪比阿托品对肠黏膜的损伤作用小,建议在治疗有机磷中毒时,宜考虑使用宾赛克嗪。
Objective To study the comparative effects of benthiazine (new antitoxic agent) and atropine on morphological changes of intestinal mucosa epithelial cells, and to determine the basis of drug selection under the clinical treatment dose of organophosphorus pesticide poisoning. Methods Forty-two healthy male Kunming mice were randomly divided into normal saline group, beimizide group and atropine group (drug control group), and the latter two groups were divided into three dose groups (2,6 and 18 mg / kg ) Were injected intraperitoneally at 0, 2, 4, 8, 10, 12, 24, 48, 72, 96, 120 and 168 h before sacrifice. The entire colon and ileum at the end of the cecum were taken, and the morphological changes of the intestinal epithelial cells in the colon and ileum were observed under a light microscope. Results 2 mg / kg dose group (beijectine and atropine): ileum villi was relatively flat, a small amount of inflammatory exudation in the thickening of the colon myometrium; 6 mg / kg dose group: ileum and colon showed a small amount of eosinophils Infiltration, submucosal edema, muscular thickening; 18 mg / kg dose group: ileum and colon showed severe exudate edema, lymphoid hyperplasia, infiltration of inflammatory cells, including intestinal ileum abscess, a small amount of epithelial cells shedding and necrosis Wait. Conclusion Both drugs have a damaging effect on the intestinal mucosa. The greater the dose, the more serious damage to the intestinal mucosa. However, in the same clinical treatment dose, the effect of bezotocetine on amebic mucosal lesion is less, and it is suggested that when using organophosphorus poisoning, it is advisable to use bezacazine.