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蛋白质二硫键异构酶既能抑制肿瘤细胞凋亡,又能促进肿瘤细胞浸润转移,在肿瘤的发展进程中具有关键作用。为了研究中药莲子心是否对该酶活性有影响,该文首先对莲子心的化学成分进行了提取分离,得到12个单体化合物,其中N-甲基乌药碱、山柰酚、金圣草素-7-O-新橘皮糖苷和甘露醇为首次从该药材中分离得到。随后该文研究了莲子心中主要的生物碱成分N-甲基乌药碱、莲心碱、异莲心碱和甲基莲心碱对蛋白质二硫键异构酶活性的影响,首次发现它们均具有抑制蛋白二硫键异构酶活性的作用,其中N-甲基乌药碱活性最强(IC501.4μmol·L~(-1))。
Protein disulfide isomerase can inhibit tumor cell apoptosis, but also promote the invasion and metastasis of tumor cells in the process of tumor development has a key role. In order to study whether the lotus root of Chinese herbal medicine has an influence on the enzyme activity, the chemical constituents of the lotus root were firstly extracted and separated to obtain 12 monomer compounds, of which N-methyl aconitine, kaempferol, Su-7-O-neo-tannin and mannitol were isolated from this medicinal material for the first time. Subsequently, the paper studied the main alkaloids in lotus heart N-methyl acne, linseed, isolins and neferine on protein disulfide isomerase activity, for the first time found that they all have inhibitory protein Disulfide isomerase activity, of which N-methyl black alkali the strongest activity (IC501.4μmol · L ~ (-1)).