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目的:探讨血管内皮生长因子(VEGF)和癌基因p53在卵巢上皮癌表达的相关性及其与卵巢上皮癌临床病理因素的关系以及对卵巢癌患者预后的影响。方法:用免疫组化二步法检测73例卵巢上皮肿瘤组织中VEGF、p53蛋白的表达(良性上皮瘤24例,交界性上皮瘤7例,上皮性癌42例)。结果:VEGF在卵巢上皮肿瘤组织中的表达,恶性组阳性表达率(86.36 %)明显高于交界性组(66.67 %)及良性组(37.50 %)(P<0.005);在恶性组中,VEGF表达在不同的病理分级、临床分期中无明显差异(P>0.05)。p53在卵巢上皮肿瘤组织中的表达,恶性组阳性表达率(76.19 %)明显高于交界性组(28.57 %)及良性组(9.09 %)(P<0.01)。在恶性组中临床晚期患者p53阳性表达率(89.29 %)高于早期患者(50.00 %)(P<0.01);在不同的病理分级中p53阳性表达率无明显差异。VEGF与p53在卵巢上皮癌中的表达呈正相关,(r =0.571 4,P<0.01)。VEGF、p53表达双阴性患者的3 a生存率66.7 %(2/3)明显高于VEGF 、p53表达双阳性患者20.00 %(5/25)。结论:VEGF与p53表达呈正相关,和卵巢癌生物学特性有关,可作为估计卵巢癌预后的重要因素。
Objective: To investigate the correlation between the expression of vascular endothelial growth factor (VEGF) and oncogene p53 in ovarian epithelial carcinoma and its relationship with the clinicopathological factors and the prognosis of patients with ovarian cancer. Methods: The expression of VEGF and p53 protein in 73 cases of ovarian epithelial tumor tissues were detected by immunohistochemical method (24 cases of benign epithelial tumor, 7 cases of borderline epithelial tumor and 42 cases of epithelial carcinoma). Results: The positive rate of VEGF expression in ovarian epithelial tumor was 86.36%, which was significantly higher than that in borderline (66.67%) and benign (37.50%) (P <0.005). In malignant group, the expression of VEGF Expression in different pathological grade, clinical stage no significant difference (P> 0.05). The positive expression rate of p53 in ovarian epithelial tumor was 76.19% in malignant group and 28.57% in benign group and 9.09% in benign group (P <0.01). In the malignant group, the positive rate of p53 (89.29%) in patients with advanced clinical stage was higher than that in early stage (50.00%) (P <0.01). There was no significant difference in the positive rate of p53 in different pathological grades. The expression of VEGF and p53 in ovarian epithelial carcinoma was positively correlated (r = 0.571 4, P <0.01). The 3-year survival rate of double-negative patients with VEGF and p53 expression was 66.7% (2/3) significantly higher than that of VEGF and 20.00% (5/25) patients with double positive p53 expression. Conclusion: There is a positive correlation between the expression of VEGF and p53 and the biological characteristics of ovarian cancer, which may be used as an important factor in estimating the prognosis of ovarian cancer.