论文部分内容阅读
目的 评价不同损毁程度 Ｃ５７ Ｂ Ｌ 小鼠帕金森病（ Ｐ Ｄ）模型纹状体多巴胺转运蛋白（ Ｄ Ａ Ｔ）的变化。方法 根据腹腔注射 Ｍ Ｐ Ｔ Ｐ 的天数将小鼠分为 １、３、５ 和 ７ｄ 模型组以及对照组，静脉注射９９ｍ Ｔｃ Ｔ Ｒ Ｏ Ｄ Ａ Ｔ１６ｍ Ｃｉ，１ｈ 后处死行脑纹状体放射自显影，同时行免疫组化酪氨酸羟化酶（ Ｔ Ｈ）染色。结果 对照组的放射自显影可见９９ｍ Ｔｃ Ｔ Ｒ Ｏ Ｄ Ａ Ｔ１ 于纹状体部位有高度放射性聚集，且两侧纹状体基本相同。注射 Ｍ Ｐ Ｔ Ｐ １ｄ 者，其纹状体的放射性浓集比对照组有所下降。注射 Ｍ Ｐ Ｔ Ｐ ３、５ 及 ７ｄ 者，两侧纹状体的放射性浓集逐日降低，第 ７ｄ 者几乎消失。 Ｔ Ｈ 染色发现黑质 Ｔ Ｈ 阳性神经元亦随注射 Ｍ Ｐ Ｔ Ｐ 天数的增加而数量减少。结论 不同程度损毁的 Ｃ５７ Ｂ Ｌ 小鼠 Ｐ Ｄ 模型可模拟 Ｐ Ｄ 的发展过程，９９ｍ Ｔｃ Ｔ Ｒ Ｏ Ｄ Ａ Ｔ１ 作为 Ｄ Ａ Ｔ 的显影剂可用于早期诊断的神经显像学研究。
Objective To evaluate the changes of dopamine transporter (D A T) in the striatum of Parkinson ’s disease (P D) mice with different degrees of damage in C57BL mice. Methods According to the number of days of intraperitoneal injection of M P T P, mice were divided into 1, 3, 5 and 7 d model group and control group, 99m Tc T R O D T 16m Ci was injected intravenously, Body autoradiography, simultaneous immunohistochemical tyrosine hydroxylase (T H) staining. Results autoradiography in the control group shows 99m Tc T R O D A T 1 highly radioactive accumulation in the striatum, and both sides of the striatum is basically the same. Compared with the control group, the radioactive concentration of striatum decreased in those injected with M P T P 1d. On the 3rd, 5th and 7th days after M P T P injection, the radioactive concentration of striatum on both sides decreased day by day, almost disappeared on the 7th day. T H staining showed that the number of substantia nigra T H-positive neurons decreased with the increase of M P T P injection. Conclusion C57BL mice with varying degrees of damage can model the development of P D. The 99m Tc-T R O D A T-1 as a D A T developer can be used in the early diagnosis of neuroimaging studies.