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用营养液培养的方法 ,以甜研 7号为试验材料 ,研究了同一氮素水平不同氮素形态比例对甜菜氮、糖代谢关键酶 :硝酸还原酶 (NR)、谷氨酰胺合成酶 (GS)和蔗糖合成酶 (SS)活性及相关产物的影响。结果表明叶片中 NR活性随NO-3比例增加而增加 ;根和叶片中 GS活性 ,当 NH+4小于 NO-3时 ,随 NH+4比例增大而增加 ,当 NO-3∶ NH+4为 1∶ 1时活性最大 ,当 NH+ 4大于 NO- 3时活性下降 ;根中 α-氨基态氮和蛋白质含量的变化与 GS活性的变化基本一致 ;生育后期 NH+ 4和 NO- 3混合培养的甜菜根中 SS合成方向的活性明显高于单一氮营养的 ,蔗糖的变化与之相对应 ;而葡萄糖和果糖则随 NH+4浓度增加而降低。块根中含糖率随 NH+4浓度增加而增加 ,块根产量和产糖量以 NO-3∶ NH+4为1∶ 1时最高
Using the method of nutrient solution culture, sweety 7 was used as the experimental material to study the effects of different nitrogen forms with the same nitrogen level on the nitrogen and sugar metabolism key enzymes of sugar beet, nitrate reductase (NR) and glutamine synthetase (GS) ) And sucrose synthase (SS) activity and related products. The results showed that the activity of NR in leaves increased with the increase of NO-3 ratio. The GS activity in roots and leaves increased with the increase of NH + 4 when NH + 4 was less than NO-3. When NO-3: NH + 4 1 at 1: 1, and decreased when the NH + 4 concentration was greater than that of NO-3. The change of α-amino nitrogen and protein content in root was consistent with that of GS activity. During the later growth period, the mixed culture of NH + 4 and NO 3 The activity of SS synthesis in beetroot was significantly higher than that of single nitrogen nutrition, and the change of sucrose corresponded to it. However, the glucose and fructose decreased with the increase of NH + 4 concentration. The sugar content in root tuber increased with the increase of NH + 4 concentration, and the highest root tuber yield and sugar content were NO: 3: NH + 4