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细胞毒性T淋巴细胞(CTL)识别经主要组织相容性抗原(MHC)提呈的病毒抗原后,能不可逆地杀伤病毒感染细胞,从而导致严重的疾病。设计一分子结构阻断CTL与感染细胞的相互作用,进而抑制CTL的活性具有重要意义。作者在25℃培养RMA-S(H-2~b)细胞表达MHC D~b分子,加入淋巴细胞脉络丛脑膜炎病毒(LCMV)GP277-286(GVENPGGVCL)及NP397～407(QPQNGQFIHFY)合成肽后,MHC D~b分子的表达持续稳定,而无该肽段D~b分子表达则迅速下降。用~(125)Ⅰ标记GP277～286及NP397407发现两者的结合为特异性;用D~b或K~b MeAb作免疫沉淀反应,表明该结合仅发生于D~b水平。用上述两肽包被RMA-S细
Cytotoxic T lymphocytes (CTLs) recognize viral antigens presented by major histocompatibility antigens (MHC) and can irreversibly kill virus-infected cells, leading to serious disease. It is of great significance to design a molecular structure to block the interaction between CTL and infected cells and then inhibit the activity of CTL. After culturing RMA-S (H-2 ~ b) cells at 25 ℃ and expressing MHC D ~ b molecules, the peptides were synthesized by adding LCMV GP277-286 (GVENPGGVCL) and NP397 ~ 407 (QPQNGQFIHFY) , The expression of MHC D ~ b molecules continued to be stable, whereas the expression of D ~ b molecules without the peptide decreased rapidly. The combination of GP277 ~ 286 and NP397407 with ~ (125) Ⅰ was found to be specific; the immunoprecipitation with D ~ b or K ~ b MeAb indicated that the binding occurred only at D ~ b level. RMA-S fine was coated with the above two peptides