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目的建立高效液相色谱-蒸发光散射检测法测定磷霉素钙胶囊含量。方法用PrevailC18(4.6mm×250mm,5μm)色谱柱,以0.25mol/L三氟乙酸水溶液为流动相,流速0.7mlm/in,蒸发光散射检测器的漂移管温度105℃,载气流速2.8Lm/in。结果线性范围0.9～2.4mgm/l,r=0.9997,平均回收率为103.1%,RSD=1.2%(n=9)。结论本法简便、快速,结果准确可靠,重现性好,可用于磷霉素钙胶囊的质量控制。
Objective To establish a method for the determination of fosfomycin calcium capsules by high performance liquid chromatography with evaporative light scattering. Methods A Prevail C18 (4.6 mm × 250 mm, 5 μm) column was used. The mobile phase consisted of a 0.25 mol / L trifluoroacetic acid solution with a flow rate of 0.7 mlm / in. The evaporative light scattering detector had a drift tube temperature of 105 ° C and a carrier gas flow rate of 2.8 Lm / in. Results The linear range was 0.9-2.4 mgm / l, r = 0.9997. The average recovery was 103.1% with RSD = 1.2% (n = 9). Conclusion The method is simple, rapid, accurate and reliable, reproducible, and can be used for quality control of Fosfomycin calcium capsules.