论文部分内容阅读
目的分别制备抗幽门螺杆菌(H.pylori)全菌(SS1株)和尿素酶I和尿素酶B的重组蛋白(r IB)的特异性卵黄抗体(Ig Y),纯化后研究其体外抑菌效果。方法分别以超声破碎SS1菌液和r IB为抗原免疫来杭鸡,用水稀释法和硫酸铵沉淀法纯化获得相应特异性Ig Y(SS1-Ig Y、IB-Ig Y)。将特异性Ig Y与SS1混合作用后共培养检测其抑菌效果,快速脲酶试验检测其抗尿素酶作用。结果成功制备2种特异性Ig Y:SS1-Ig Y和IB-Ig Y,效价超过1∶12 800,纯度超过80%。SS1-Ig Y和IB-Ig Y均有体外抗尿素酶活性和抑菌作用。结论 SS1-Ig Y和IB-Ig Y均具有在体外抑制H.pylori尿素酶活性和抑制H.pylori生长的作用。
OBJECTIVE To prepare specific IgY antibodies against the recombinant protein (r IB) of Helicobacter pylori (SS1) and urease I and urease B effect. Methods The specific Ig Y (SS1-Ig Y, IB-Ig Y) was purified from the suspension of SS1 and rBB by immunoblotting with antigens and dilution with water and ammonium sulfate precipitation. The specific Ig Y mixed with SS1 after co-culture to detect its antibacterial effect, rapid urease test to detect its anti-urease action. Results Two specific Ig Y: SS1-Ig Y and IB-Ig Y were successfully prepared. The titer was over 1:12 800 and the purity was over 80%. Both SS1-Ig Y and IB-Ig Y had anti-urease activity and antibacterial activity in vitro. Conclusion Both SS1-Ig Y and IB-Ig Y have the inhibitory effect on H.pylori urease activity and the inhibition of H.pylori growth in vitro.