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牻牛儿牻牛儿基焦磷酸合酶(GGPPS)是萜烯类物质合成的关键酶之一,推测与树脂合成能力相关.本研究根据马尾松GGPPS基因设计荧光定量引物,检测不同单株中基因表达水平,并与其产脂力进行相关性分析,结果显示两者相关系数为0.733,在0.01水平上显著相关.同时,将马尾松GGPPS基因的正义、反义片段分别转化拟南芥,检测发现转化正义片段的拟南芥植株中可检测到外源GGPPS基因表达,并且叶片中两种主要的二萜组分含量较野生型显著提高.转化反义片段的植株中,内源GGPPS基因表达则受到显著抑制,多种二萜组分含量显著降低.拟南芥转化结果说明GGPPS是二萜组分合成过程中的重要基因,并且马尾松GGPPS正义片段的转化能显著地促进二萜组分的合成.本研究结果为高产脂马尾松选育的分子辅助育种提供了有益的技术参考.“,”GGPPS (geranylgeranyl pyrophosphate synthases, GGPPS) is one of the key enzymes in the synthesis of terpenes, which is speculated to be related to the synthesis ability of resin.In this study, we designed fluorescent quantitative primers according to the GGPPS gene of Pinus massoniana, detected the gene expression level in different individuals, and analyzed its correlation with the resin producing capacity.The results showed that the correlation coefficient of the two was 0.733, which was significantly correlated at the 0.01 level (p＜0.01).Meanwhile, we transformed the sense and anti-sense segments of Pinus massoniana GGPPS gene into Arabidopsis thaliana, respectively, and found that the expression of exogenous GGPPS gene could be detected in Arabidopsis thaliana plants transformed with the sense fragment.Moreover, the contents of two major diterpenoids in the leaves were significantly higher than those in wild type.However, in the plants transformed with the anti-sense fragment, the expression of the endogenous GGPPS gene was significantly inhibited, and the contents of various diterpenoids were significantly reduced.The transformation results in Arabidopsis thaliana indicated that GGPPS was a critical gene functioning in the synthesis process of diterpenoids, and the transformation of the sense fragment of Pinus massoniana GGPPS gene could significantly promote the synthesis of diterpenes.This study might provide useful technical references for the molecular assisted breeding of Pinus massoniana with high-yield resin.