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目的 研究大鼠肝星状细胞( H S C) L D L、 H D L 受体。方法 用链霉蛋白酶和胶原酶原位灌流, Nycodenz 密度梯度离心分离大鼠 H S C;应用125 I L D L 和125 I H D L3 配体进行放射性配基结合实验测定 H S C L D L、 H D L 受体。结果 H S C 膜表面存在 L D L、 H D L 受体, H S C 与浓度递增的125 I L D L 和125 I H D L3 共同孵育进行饱和试验, H S C L D L、 H D L 受体特异性结合 L D L、 H D L 的浓度效应曲线,呈可饱和性指数曲线,将特异结合用于 Scatchard 作图,r 分别为- 0 .9751 和- 0 .9616 ,解离常数分别为8 .4736n mol/ L 和9 .1256n mol/ L,最大结合容量分别为439 .32 和259 .34fmol/mg 蛋白。结论 H S C L D L、 H D L 受体对脂蛋白代谢及胆固醇的调节有重要作用,为阐明脂肪肝肝纤维化的发生提供了新的实验依据。
Objective To study rat hepatic stellate cells (H S C) L D L and H D L receptors. Methods In situ perfusion with pronase and collagenase, and centrifugation of rat H S C by Nycodenz density gradient centrifugation were performed using 125 I-L D and 125 I-H D L3 ligands for determination of H S C L D L, H D L receptor. Results There was L D L and H D L receptors on the surface of H S C membrane. H S C was incubated with 125 I-L D L and 125 I H D L 3 with increasing concentrations. H S C L D L, H D L receptor specific binding L D L, H D L concentration effect curve, saturability index curve will be used for specific binding Scatchard plot, r were -0. 9751 and - 0. 9616, the dissociation constants are 8 respectively. 4736 n mol / L and 9. 1256n mol / L, the maximum binding capacity of 439 respectively. 32 and 259. 34 fmol / mg protein. Conclusion H S C L D L and H D L receptors play an important role in the metabolism of lipoproteins and the regulation of cholesterol. It provides a new experimental basis for elucidating the occurrence of hepatic fibrosis in fatty liver.