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目的:探讨疑核对炎症性肠病(inflammatory bowel disease,IBD)相关细胞因子IL-6的调控作用,以及在IBD发生发展过程中发挥的作用.方法:三硝基苯磺酸(TNBS)诱导IBD大鼠动物模型,采用脑立体定位技术和电生理方法,应用Real-time PCR方法来检测急性电刺激疑核前后IBD大鼠结肠、胸腺、脾脏、外周血淋巴细胞中IL-6mRNA表达水平的变化.结果:经急性电刺激疑核后,IBD模型大鼠结肠、胸腺、脾脏及外周血淋巴细胞中IL-6的mRNA转录水平均显著升高(3.26E-02±5.83E-03vs5.12E-03±6.86E-04,5.52E-02±9.56E-03vs2.04E-02±3.58E-03,3.42E-02±7.19E-03vs2.91E-03±3.41E-04,8.12E-03±4.24E-04vs3.74E-03±5.73E-04,均P<0.01),而假刺激组和对照组没有明显变化(P>0.05).结论:疑核对IBD大鼠相关细胞因子IL-6具有一定的调控作用,且以正调控作用为主,并且在IBD发生发展过程中可能发挥着重要的作用.
OBJECTIVE: To investigate the regulatory effect of suspicion on IL-6 related to inflammatory bowel disease (IBD) and its role in the development and progression of IBD.Methods: Trinitrobenzene sulfonic acid (TNBS) induced IBD Rat model, using stereotactic and electrophysiological methods, Real-time PCR was used to detect the expression of IL-6mRNA in colon, thymus, spleen and peripheral blood lymphocytes of IBD rats before and after suspected electric shock Results: After acute electrical stimulation of suspicious nuclei, mRNA transcription levels of IL-6 in colon, thymus, spleen and peripheral blood lymphocytes in IBD model rats were significantly increased (3.26E-02 ± 5.83E-03vs5.12E- 03 ± 6.86E-04,5.52E-02 ± 9.56E-03vs2.04E-02 ± 3.58E-03,3.42E-02 ± 7.19E-03vs2.91E-03 ± 3.41E-04,8.12E-03 ± 4.24E-04vs3.74E-03 ± 5.73E-04, all P <0.01), while there was no significant difference between the sham group and the control group (P> 0.05) .Conclusion: Susceptibility to IL- A certain degree of regulation and control, and the main role of positive regulation, and in the occurrence and development of IBD may play an important role.