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目的 :研究同种异体肾上腺髓质植入蛛网膜下腔对慢性痛模型大鼠痛行为的影响及其发生机制。方法 :将同种大鼠肾上腺髓质嗜铬细胞异体移植入脊髓背角蛛网膜下腔 ,6w后进行组织学检查 ,并用高压液相色谱法检测脑脊液中嗜铬细胞释放的肾上腺素 (E) ,同时观察移植的嗜铬细胞在 1～ 8w内减轻痛行为的表现。结果 :移植 6w后 ,嗜铬细胞浆内有明显的嗜铬颗粒 ,分泌的肾上腺素明显高于对照组。移植后 1～ 8w内动物的屈关节嘶叫反应评分、关节的肿胀程度、体重的减轻程度 ,移植组均明显低于对照组 (P <0 0 5 )。结论 :移植的嗜铬细胞在蛛网膜下腔能够存活并释放肾上腺素镇痛活性物质 ,减轻慢性痛模型大鼠的痛表现。
Objective: To study the effect and mechanism of allogeneic adrenal medulla implantation on the pain behavior of chronic pain model rats. Methods: Allograft rat adrenal medullary chromaffin cells were allotransplanted into the subarachnoid space of the dorsal horn of the spinal cord. Histological examination was performed 6 weeks later. The epinephrine (E) released from the chromaffin cells in cerebrospinal fluid was detected by high pressure liquid chromatography. , While observing the transplanted chromaffin cells in 1 ~ 8w to reduce the behavior of pain behavior. Results: After 6 weeks of transplantation, there was obvious chromaffin particles in the pheochromocytoplasm, and the secretion of epinephrine was significantly higher than that of the control group. The scores of flexion of the joints, the degree of swelling of the joints and the degree of weight loss within 1 ~ 8w after transplantation were significantly lower in the transplantation group than those in the control group (P <0.05). CONCLUSION: The transplanted chromaffin cells can survive in the subarachnoid space and release the epinephrine analgesic active substance, and relieve the pain performance of chronic pain model rats.