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为了观察创面愈合过程中转化生长因子－β１（ＴＧＦ－β１）的基因表达，以大鼠断层供皮区创面为模型，应用原位杂交、斑点杂交等方法对内源性ＴＧＦ－β１ｍＲＮＡ在创面愈合过程中的表达变化进行了观察。结果表明，ＴＧＦ－β１ｍＲＮＡ主要在成纤维细胞、上皮细胞及毛细血管内皮细胞中表达，其表达量在伤后第６天达峰值，以后随上皮化的完成，ＴＧＦ－β１ｍＲＮＡ表达水平逐渐降至正常。提示，在创面愈合过程中ＴＧＦ－β１基因有明显表达，其表达规律与创面愈合时间有密切关系。认为，ＴＧＦ－β１可能在创面愈合过程中起着重要的内在调控作用
In order to observe the gene expression of transforming growth factor-β1 (TGF-β1) during wound healing, the wounds of rat donor grafts were used as models. The wound healing of endogenous TGF-β1 mRNA was detected by in situ hybridization and dot blot hybridization The process of expression changes were observed. The results showed that TGF-β1 mRNA was mainly expressed in fibroblasts, epithelial cells and capillary endothelial cells. The expression of TGF-β1 mRNA peaked on the 6th day after injury, and gradually decreased to normal with the completion of epithelialization . Tip, in the process of wound healing TGF-β1 gene was significantly expressed in the expression of wound healing time is closely related. It is believed that TGF-β1 may play an important role in the process of wound healing