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目的利用MS-HRM技术建立Syk(L)基因启动子甲基化定量检测的方法,并探讨其甲基化程度与喉鳞癌临床病理参数的相关性。方法提取48份喉鳞癌组织及20份癌旁正常喉黏膜组织DNA并进行甲基化修饰。用MSHRM技术定量检测Syk(L)基因启动子甲基化水平,并分析评价其与喉鳞癌临床病理参数的相关性。结果 MS-HRM检测Syk(L)基因启动子甲基化结果为20份癌旁正常喉黏膜组织均位于0%区域;喉鳞癌组织中6份位于0%区域,8份位于0%~30%区域,20份位于30%~70%区域,14份位于70%~100%区域。Syk(L)基因启动子甲基化水平与喉鳞癌临床分型差异无统计学意义(P>0.05),但与临床T分期、病理组织学分级、颈淋巴结转移差异有统计学意义(P<0.05),且Syk(L)甲基化水平随临床T分期及病理组织学分级增高而增高。结论 MS-HRM定量检测Syk(L)基因启动子甲基化有望成为喉鳞癌进展评估分子指标检测的有效手段。
OBJECTIVE: To establish a method for the quantitative detection of Syk (L) promoter methylation using MS-HRM and to explore its correlation with the clinicopathological parameters of LSCC. Methods DNA was extracted from 48 samples of laryngeal squamous cell carcinoma and 20 normal laryngeal squamous cell carcinoma tissues and methylated. The methylation level of Syk (L) gene promoter was quantitatively determined by MSHRM technique and its correlation with clinicopathological parameters of laryngeal squamous cell carcinoma was analyzed. Results Methyltransferase methylation of Syk (L) gene was detected in 0% of normal laryngeal squamous cell carcinoma tissues by MS-HRM, 6% of laryngeal squamous cell carcinoma tissues and 0% ~ 30 of laryngeal squamous cell carcinoma tissues % Of the area, 20 in the 30% ~ 70% area, 14 in the 70% ~ 100% area. The methylation level of Syk (L) gene was not significantly different from that of laryngeal squamous cell carcinoma (P> 0.05), but was significantly different from clinical stage, pathological grade and cervical lymph node metastasis <0.05), and the Syk (L) methylation level increased with the clinical T stage and histopathological grade. Conclusion MS-HRM quantitative detection of Syk (L) gene promoter methylation is expected to be an effective means for the detection of molecular markers for the progression of laryngeal squamous cell carcinoma.