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研究了用~(111)In通过DTPA环二酸酐标记单克隆抗体(McAb)的各个实验环节,制备了超纯~(111)In,得到了高比活度1.11GBq/mgMcAb、具有免疫反应活性的~(111)In-McAb。对用~(111)In标记的抗CEA McAb C14-C50、抗人结肠癌McAb 2C10和鼠IgG在荷瘤裸鼠模型体内分布及体外显象进行了研究。注射单抗的动物得到清晰的肿瘤显象,而注射普通IsG的动物的肿瘤不显象。
In this paper, we have studied the various experimental steps of labeling (111) In with DTPA cyclic dianhydride and monoclonal antibody (McAb). The ultrapure ~ (111) In was prepared and the high specific activity of 1.11GBq / mgMcAb was obtained. ~ (111) In-McAb. The in vivo distribution and in vitro imaging of ~ (111) In-labeled anti-CEA McAb C14-C50, anti-human colon cancer McAb 2C10 and murine IgG in nude mice were studied. Animals injected with mAb gave clear tumor imaging whereas tumors injected with normal IsG did not develop tumors.