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目的建立两株新建的肝癌细胞系并观察其生物学特性。方法采用HE染色技术、细胞计数法、琼脂糖电泳法、ELISA、流式细胞仪、核型分析和异种移植等对两株新的肝癌细胞系进行生物学特性研究。结果两株细胞分别命名为LXY-1、XMS-1,均已获得长期体外生长的能力,无论形态学观察,还是LDH同工酶谱的变化,均符合肝癌细胞的一般特征,细胞培养上清及胞质中均不分泌AFP、HBsAg。XMS-1细胞DNA整合有HBV DNA,染色体平均数分别为:LXY-1105条、XMS-150条。两株细胞S、G2-M期细胞比例增加,细胞倍增时间介于19.5～59.2h之间,异种移植成瘤率:LXY-175%,XMS-1不成瘤。结论两株肝癌细胞均具有人肝癌细胞的一般特征,可用于肝癌的防治研究。
Objective To establish two new hepatoma cell lines and observe their biological characteristics. Methods HE staining, cell counting, agarose gel electrophoresis, ELISA, flow cytometry, karyotyping and xenograft were used to study the biological characteristics of two new hepatocellular carcinoma cell lines. Results The two cells were named as LXY-1 and XMS-1, respectively. All of them had obtained long-term growth in vitro. The morphological observation and the change of LDH isoenzymes were in line with the general characteristics of hepatocellular carcinoma cells. And the cytoplasm are not secreting AFP, HBsAg. The DNA of XMS-1 cells was integrated with HBV DNA. The average number of chromosomes was LXY-1105 and XMS-150, respectively. The proportion of cells in S and G2-M phase of two cells increased, the cell doubling time ranged from 19.5 to 59.2 h, the rate of xenograft tumorigenesis was LXY-175%, and XMS-1 did not form tumor. Conclusion Both hepatocellular carcinoma cells have the general characteristics of human hepatoma cells and can be used for prevention and treatment of liver cancer.